Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 4 | 15.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HIICAGJF_1 | 2555953 | 2556807 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| HIICAGJF_1 | 2537159 | 2538361 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| HIICAGJF_1 | 2365869 | 2367038 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| HIICAGJF_1 | 1439558 | 1439875 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| HIICAGJF_1 | 1439220 | 1439558 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| HIICAGJF_1 | 944020 | 946590 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| HIICAGJF_1 | 391461 | 392054 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| HIICAGJF_1 | 343571 | 345004 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HIICAGJF_1 | 2555955 | 2556807 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| HIICAGJF_1 | 317302 | 318221 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HIICAGJF_1 | 2555956 | 2556807 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| HIICAGJF_1 | 4040293 | 4040811 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| HIICAGJF_1 | 2058536 | 2061133 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
| HIICAGJF_1 | 651323 | 652354 | vmlR | ABC-F type ribosomal protection protein VmlR | PARTIALX | 97.38 | 62.71 | WP_003234144.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HIICAGJF_1 | 3539952 | 3541094 | capA | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | NP_391469 |
| HIICAGJF_1 | 3114137 | 3115075 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| HIICAGJF_1 | 3541577 | 3542758 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| HIICAGJF_1 | 3541113 | 3541562 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| HIICAGJF_1 | 2166511 | 2167152 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| HIICAGJF_1 | 3117962 | 3118747 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| HIICAGJF_1 | 2007779 | 2009542 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| HIICAGJF_1 | 3116740 | 3117936 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| HIICAGJF_1 | 3106981 | 3114117 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.19 | 100.00 | NP_391076 |
| HIICAGJF_1 | 3115103 | 3116722 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HIICAGJF_1 | 132584 | 133720 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| HIICAGJF_1 | 18769 | 19638 | pdxS | 3.03e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| HIICAGJF_1 | 1784192 | 1784968 | CodY | 3.30e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| HIICAGJF_1 | 3380865 | 3381434 | ClpP | 2.45e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HIICAGJF_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HIICAGJF_1 | 180425 | 214252 | False | Myoviridae | VOG10058, VOG0198, VOG6242, VOG4618, VOG4567, VOG1328, VOG5361, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG10120, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705, VOG0753 |
| HIICAGJF_1 | 1383794 | 1409733 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)