safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	0	1	1.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
LIFKBJCB_1	1725358	1724657	WalR	4.24e-107	transcriptional regulatory protein	80.34	99.57	EPH95667

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
LIFKBJCB_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
LIFKBJCB_1	171737	204154	False	Siphoviridae	VOG0283, VOG0226, VOG0514, VOG4566, VOG0189, VOG1931, VOG0044, VOG0044, VOG3643, VOG0819, VOG0149, VOG0198, VOG6229, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209, VOG0660, VOG4910, VOG4605, VOG4599, VOG10972, VOG9955
LIFKBJCB_1	264151	304109	False	Myoviridae	VOG9706, VOG1309, VOG0186, VOG4855, VOG7236, VOG0044, VOG3643, VOG4693, VOG6005, VOG0198, VOG1315, VOG4214, VOG5343, VOG0052, VOG7407, VOG0720, VOG4564, VOG3434, VOG1887, VOG2621, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG4910, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG10914, VOG3298, VOG3298
LIFKBJCB_1	683343	711613	False	Myoviridae	VOG4845, VOG4691, VOG0573, VOG1348, VOG1433, VOG1956, VOG4910, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4710, VOG1887, VOG1887, VOG3434, VOG4555, VOG4564, VOG0720, VOG4544, VOG2545, VOG0376, VOG0275, VOG0796, VOG0198, VOG0586
LIFKBJCB_1	714515	722903	False	Siphoviridae	VOG0536, VOG0044, VOG1177, VOG6005, VOG0514, VOG0025, VOG4799, VOG6545, VOG0322, VOG0685
LIFKBJCB_1	727179	734833	True	Unknown	VOG4552, VOG6495, VOG0862, VOG1561, VOG0275, VOG10391, VOG4549, VOG5088
LIFKBJCB_1	877502	889215	False	Siphoviridae	VOG3477, VOG0198, VOG0204, VOG4553, VOG4556, VOG5051, VOG4544, VOG4581, VOG4841, VOG0205, VOG4609
LIFKBJCB_1	2009100	2010982	False	Unknown	VOG4566
LIFKBJCB_1	2449852	2488590	False	Myoviridae	VOG6421, VOG4602, VOG6495, VOG9667, VOG0787, VOG1638, VOG5353, VOG0650, VOG1309, VOG0186, VOG4566, VOG4552, VOG0044, VOG3643, VOG4693, VOG6005, VOG0198, VOG1315, VOG0045, VOG4699, VOG0611, VOG7407, VOG8453, VOG0720, VOG4564, VOG1329, VOG5795, VOG1353, VOG1883, VOG1352, VOG4699, VOG4910, VOG4865, VOG1433, VOG1348, VOG0573, VOG2349, VOG4691, VOG4845
LIFKBJCB_1	2758632	2778102	False	Siphoviridae	VOG6888, VOG0321, VOG0283, VOG0226, VOG10904, VOG0514, VOG4566, VOG0052, VOG3480, VOG8425, VOG0044, VOG0198, VOG6725, VOG4214, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209