Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 1 | 23 | 31 | 38.61 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DIHAIPFB_48 | 866 | 2806 | tet(S) | ARO:3000192 | tet(S) | 99.84 | 100.78 | L09756.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DIHAIPFB_48 | 866 | 2791 | tet(S) | Doxycycline, Tetracycline, Minocycline | 100.00 | 100.00 | L09756 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DIHAIPFB_48 | 869 | 2791 | tet(S) | tetracycline resistance ribosomal protection protein Tet(S) | EXACTX | 100.00 | 100.00 | WP_000691722.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DIHAIPFB_24 | 16522 | 17331 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| DIHAIPFB_11 | 121167 | 122310 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.65 | 100.00 | - |
| DIHAIPFB_12 | 13082 | 14740 | fbp54 | 0.0 | FBPs | Adherence | 99.34 | 100.00 | WP_011227179 |
| DIHAIPFB_25 | 11873 | 13333 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 99.25 | 100.00 | WP_011681177 |
| DIHAIPFB_24 | 15317 | 16522 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.25 | 100.00 | WP_011226326 |
| DIHAIPFB_24 | 18268 | 19416 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.22 | 100.00 | WP_041828290 |
| DIHAIPFB_24 | 17315 | 18271 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.16 | 100.00 | WP_011226328 |
| DIHAIPFB_24 | 12463 | 14245 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.10 | 99.89 | WP_011226325 |
| DIHAIPFB_2 | 3529 | 4398 | rfbA | 0.0 | Capsule | Immune modulation | 99.08 | 100.00 | WP_011681286 |
| DIHAIPFB_18 | 399 | 1250 | rfbD | 0.0 | Capsule | Immune modulation | 99.06 | 100.00 | WP_014621820 |
| DIHAIPFB_24 | 14242 | 15297 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.05 | 100.00 | WP_002948022 |
| DIHAIPFB_2 | 2936 | 3529 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 98.99 | 100.00 | WP_011226149 |
| DIHAIPFB_2 | 2936 | 3529 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 98.82 | 100.00 | WP_011226149 |
| DIHAIPFB_2 | 2936 | 3529 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 98.82 | 100.00 | WP_011226149 |
| DIHAIPFB_25 | 13334 | 14065 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011681176 |
| DIHAIPFB_2 | 37490 | 38251 | srtA | 0.0 | Sortase A | Exoenzyme | 98.42 | 100.00 | - |
| DIHAIPFB_36 | 10405 | 11244 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.33 | 100.00 | WP_011226733 |
| DIHAIPFB_25 | 13334 | 14065 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.95 | 100.00 | WP_011226048 |
| DIHAIPFB_25 | 13334 | 14065 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011227248 |
| DIHAIPFB_25 | 11873 | 13333 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.99 | 100.21 | WP_011226049 |
| DIHAIPFB_25 | 14776 | 15516 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 96.76 | 100.00 | WP_011681174 |
| DIHAIPFB_25 | 11873 | 13333 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.58 | 100.21 | WP_041827043 |
| DIHAIPFB_25 | 14776 | 15516 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 96.36 | 100.00 | WP_011226046 |
| DIHAIPFB_25 | 14776 | 15511 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.20 | 98.13 | WP_011227246 |
| DIHAIPFB_25 | 14074 | 14766 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.96 | 100.00 | WP_011226047 |
| DIHAIPFB_25 | 14074 | 14766 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.67 | 100.00 | WP_011227247 |
| DIHAIPFB_25 | 15573 | 16938 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.95 | 99.85 | WP_011681173 |
| DIHAIPFB_25 | 15573 | 16940 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.71 | 100.00 | WP_011226045 |
| DIHAIPFB_25 | 14074 | 14766 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 92.06 | 100.00 | WP_011681175 |
| DIHAIPFB_11 | 21572 | 22875 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.02 | 99.46 | WP_000022832 |
| DIHAIPFB_28 | 253 | 1263 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.66 | 99.90 | WP_002986042 |
| DIHAIPFB_23 | 3363 | 4646 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| DIHAIPFB_2 | 1460 | 2506 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| DIHAIPFB_39 | 1471 | 2054 | SPD_RS01775 | 1.10e-145 | Capsule | Immune modulation | 82.73 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DIHAIPFB_7 | 24893 | 25966 | MntE | 0.0 | manganese homeostasis | 94.97 | 99.72 | ABF32167 |
| DIHAIPFB_2 | 40938 | 41921 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| DIHAIPFB_11 | 104887 | 103919 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| DIHAIPFB_22 | 165003 | 166484 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.51 | 100.00 | ABJ54480 |
| DIHAIPFB_19 | 11730 | 9427 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.45 | 99.22 | AAK74619 |
| DIHAIPFB_20 | 19210 | 16964 | metE | 0.0 | methionine biosynthesis enzymes | 91.72 | 100.00 | AAK74738 |
| DIHAIPFB_31 | 1884 | 886 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| DIHAIPFB_29 | 116 | 1087 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| DIHAIPFB_17 | 21955 | 23106 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| DIHAIPFB_18 | 16191 | 14203 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.14 | 100.00 | AE014259_2 |
| DIHAIPFB_2 | 25547 | 23817 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.87 | 100.00 | AAK34199 |
| DIHAIPFB_77 | 28370 | 29848 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| DIHAIPFB_11 | 22875 | 21574 | Eno | 0.0 | enolase | 85.98 | 99.77 | ABP90468 |
| DIHAIPFB_77 | 43569 | 45461 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.74 | 98.90 | ABP91129 |
| DIHAIPFB_20 | 14039 | 15748 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| DIHAIPFB_33 | 50286 | 51296 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| DIHAIPFB_5 | 34306 | 35667 | mnmE | 0.0 | central tRNA-modifying GTPase | 81.80 | 99.56 | ABP90420 |
| DIHAIPFB_11 | 37305 | 36415 | bglB | 1.25e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| DIHAIPFB_1 | 46677 | 47615 | SP_0676 | 6.00e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| DIHAIPFB_20 | 16772 | 15915 | metF | 7.34e-167 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| DIHAIPFB_44 | 82402 | 83106 | covR | 2.57e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| DIHAIPFB_22 | 131212 | 130541 | rr01 | 2.73e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| DIHAIPFB_20 | 10085 | 9417 | CiaR | 9.67e-126 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| DIHAIPFB_20 | 10085 | 9414 | ciaR | 8.81e-124 | two component system response regulator | 85.27 | 99.12 | AIK75708 |
| DIHAIPFB_44 | 82402 | 83106 | walR | 1.08e-122 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| DIHAIPFB_3 | 7678 | 6992 | CovR | 7.19e-120 | serves to repress GAS virulence factor-encoding genes | 81.22 | 100.00 | ACE75886 |
| DIHAIPFB_31 | 610 | 215 | SpxA2 | 2.50e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| DIHAIPFB_6 | 25417 | 25130 | spr0479 | 1.18e-48 | essential gene | 84.38 | 98.97 | AAK99283 |
| DIHAIPFB_11 | 163740 | 163486 | spr1327 | 2.61e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| DIHAIPFB_9 | 14574 | 14837 | spr0175 | 1.80e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| DIHAIPFB_9 | 15401 | 15667 | spr0177 | 3.18e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| DIHAIPFB_23 | 4804 | 5304 | RopE (M5005_Spy_1611) | 4.47e-77 | probable DNA-directed RNA polymerase subunit delta | 82.04 | 87.43 | AAK34603 |
| DIHAIPFB_19 | 11730 | 9433 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.42 | 98.08 | MYN69560 |
| DIHAIPFB_33 | 86147 | 85128 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DIHAIPFB_1 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_2 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_3 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_4 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_5 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_6 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_7 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_8 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_9 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_10 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_11 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_12 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_13 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_14 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_15 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_16 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_17 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_18 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_19 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_20 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_21 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_22 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_23 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_24 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_25 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_26 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_27 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_28 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_29 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_30 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_31 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_32 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_33 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_34 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_35 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_36 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_37 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_38 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_39 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_40 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_41 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_42 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_43 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_44 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_45 | 1.0 | 0.0 | 1.0 |
| DIHAIPFB_46 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_48 | 1.0 | 0.0 | 1.0 |
| DIHAIPFB_49 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_50 | 1.0 | 0.0 | 1.0 |
| DIHAIPFB_51 | 1.0 | 0.0 | 1.0 |
| DIHAIPFB_52 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_53 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_54 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_55 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_56 | 1.0 | 0.0 | 1.0 |
| DIHAIPFB_57 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_58 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_59 | 1.0 | 0.0 | 1.0 |
| DIHAIPFB_60 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_61 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_62 | 1.0 | 0.0 | 1.0 |
| DIHAIPFB_63 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_64 | 1.0 | 0.0 | 1.0 |
| DIHAIPFB_65 | 1.0 | 0.0 | 1.0 |
| DIHAIPFB_66 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_67 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_68 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_69 | 1.0 | 0.0 | 1.0 |
| DIHAIPFB_71 | 1.0 | 0.0 | 1.0 |
| DIHAIPFB_72 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_77 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_78 | 0.0 | 1.0 | 0.0 |
| DIHAIPFB_79 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)