Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 7 | 17.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BBDHIEOI_14 | 1684823 | 1685161 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BBDHIEOI_14 | 1684506 | 1684823 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BBDHIEOI_14 | 697416 | 698585 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| BBDHIEOI_14 | 473222 | 474424 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| BBDHIEOI_14 | 454881 | 455735 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| BBDHIEOI_15 | 409384 | 411012 | vmlR | ARO:3004476 | vmlR | 99.08 | 99.09 | NC_000964.3 |
| BBDHIEOI_11 | 261629 | 264199 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| BBDHIEOI_15 | 153408 | 154001 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| BBDHIEOI_15 | 102846 | 104285 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| BBDHIEOI_15 | 90030 | 90950 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BBDHIEOI_14 | 454881 | 455733 | aadK | Streptomycin | 99.30 | 99.77 | M26879 |
| BBDHIEOI_13 | 1029654 | 1031030 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.91 | 100.00 | X08034 |
| BBDHIEOI_15 | 90030 | 90950 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BBDHIEOI_15 | 409387 | 411012 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.08 | 98.90 | WP_003234144.1 |
| BBDHIEOI_15 | 90030 | 90947 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| BBDHIEOI_13 | 1029657 | 1031030 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| BBDHIEOI_14 | 454881 | 455732 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| BBDHIEOI_13 | 1027183 | 1027701 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| BBDHIEOI_14 | 1003362 | 1005959 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.17 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BBDHIEOI_13 | 112923 | 113708 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| BBDHIEOI_13 | 109087 | 110025 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| BBDHIEOI_13 | 528035 | 529216 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| BBDHIEOI_13 | 526410 | 527552 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| BBDHIEOI_14 | 897317 | 897958 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| BBDHIEOI_13 | 101931 | 109067 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.37 | 100.00 | NP_391076 |
| BBDHIEOI_13 | 527571 | 528020 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| BBDHIEOI_13 | 111701 | 112897 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| BBDHIEOI_14 | 1054189 | 1055952 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| BBDHIEOI_13 | 110053 | 111672 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.33 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BBDHIEOI_10 | 31721 | 32857 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BBDHIEOI_12 | 12978 | 12109 | pdxS | 1.24e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BBDHIEOI_14 | 1374709 | 1373933 | CodY | 1.71e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BBDHIEOI_13 | 365862 | 366431 | ClpP | 6.43e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BBDHIEOI_14 | 1834946 | 1834554 | spxA1 | 1.35e-69 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BBDHIEOI_14 | 1834946 | 1834554 | spx | 8.03e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BBDHIEOI_16 | 34717 | 34475 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BBDHIEOI_15 | 356397 | 356594 | CspA | 1.90e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BBDHIEOI_9 | 0.0 | 1.0 | 0.0 |
| BBDHIEOI_10 | 0.0 | 1.0 | 0.0 |
| BBDHIEOI_11 | 0.0 | 1.0 | 0.0 |
| BBDHIEOI_12 | 0.0 | 1.0 | 0.0 |
| BBDHIEOI_13 | 0.0 | 1.0 | 0.0 |
| BBDHIEOI_14 | 0.0 | 1.0 | 0.0 |
| BBDHIEOI_15 | 0.0 | 1.0 | 0.0 |
| BBDHIEOI_16 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BBDHIEOI_14 | 504008 | 527192 | False | Myoviridae | VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| BBDHIEOI_14 | 1714962 | 1741092 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)