Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 2 | 8 | 8.49 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| LKMAKOFG_5 | 3489 | 4865 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 86.86 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LKMAKOFG_5 | 3495 | 4865 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.00 | 99.78 | WP_003242953.1 |
| LKMAKOFG_5 | 5078 | 5596 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 80.35 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| LKMAKOFG_12 | 76949 | 78130 | capB | 0.0 | Capsule | Immune modulation | 82.29 | 99.66 | NP_391471 |
| LKMAKOFG_12 | 78145 | 78594 | capC | 1.93e-113 | Capsule | Immune modulation | 83.37 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LKMAKOFG_17 | 27966 | 26830 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| LKMAKOFG_28 | 12378 | 11509 | pdxS | 1.34e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| LKMAKOFG_1 | 95092 | 94316 | CodY | 5.68e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| LKMAKOFG_9 | 50351 | 50743 | spxA1 | 2.85e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| LKMAKOFG_4 | 284515 | 284712 | CspA | 2.04e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| LKMAKOFG_3 | 410963 | 410769 | CspD | 2.69e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| LKMAKOFG_6 | 163847 | 164041 | CspB | 4.18e-27 | cold shock protein | 84.61 | 98.48 | CAD00094 |
| LKMAKOFG_4 | 284515 | 284709 | CspR | 2.67e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| LKMAKOFG_2 | 322231 | 323103 | lipA (SAUSA300_0829) | 1.48e-162 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LKMAKOFG_1 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_2 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_3 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_4 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_5 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_6 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_7 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_8 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_9 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_10 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_11 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_12 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_13 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_14 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_15 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_16 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_17 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_18 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_19 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_20 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_21 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_22 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_23 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_24 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_25 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_26 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_27 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_28 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_29 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_30 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_31 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_32 | 1.0 | 0.0 | 1.0 |
| LKMAKOFG_33 | 0.0 | 1.0 | 0.0 |
| LKMAKOFG_34 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| LKMAKOFG_1 | 469295 | 477240 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG4612, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885 |
| LKMAKOFG_26 | 4995 | 15248 | False | Siphoviridae | VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG5216, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)