Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 8 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GFBIJKNM_11 | 186735 | 187904 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| GFBIJKNM_19 | 178576 | 181146 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| GFBIJKNM_1 | 161191 | 161784 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| GFBIJKNM_1 | 113301 | 114740 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
| GFBIJKNM_4 | 87491 | 88345 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| GFBIJKNM_20 | 85717 | 86055 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| GFBIJKNM_20 | 85400 | 85717 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| GFBIJKNM_4 | 68697 | 69899 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GFBIJKNM_4 | 87493 | 88345 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| GFBIJKNM_1 | 87032 | 87951 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GFBIJKNM_4 | 87494 | 88345 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| GFBIJKNM_25 | 69966 | 70484 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| GFBIJKNM_43 | 4306 | 6903 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
| GFBIJKNM_26 | 53440 | 54471 | vmlR | ABC-F type ribosomal protection protein VmlR | PARTIALX | 97.38 | 62.71 | WP_003234144.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GFBIJKNM_39 | 2121 | 3263 | capA | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | NP_391469 |
| GFBIJKNM_13 | 73667 | 74605 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| GFBIJKNM_39 | 3746 | 4927 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| GFBIJKNM_39 | 3282 | 3731 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| GFBIJKNM_27 | 98579 | 99220 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| GFBIJKNM_13 | 69995 | 70780 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| GFBIJKNM_14 | 85303 | 87066 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| GFBIJKNM_13 | 70806 | 72002 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| GFBIJKNM_13 | 74625 | 81761 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.18 | 100.00 | NP_391076 |
| GFBIJKNM_13 | 72020 | 73639 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GFBIJKNM_36 | 32066 | 33202 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| GFBIJKNM_21 | 12176 | 11307 | pdxS | 1.23e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| GFBIJKNM_40 | 19444 | 20220 | CodY | 1.12e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GFBIJKNM_3 | 47426 | 47995 | ClpP | 7.71e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| GFBIJKNM_35 | 42668 | 42276 | spxA1 | 4.79e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| GFBIJKNM_35 | 42668 | 42276 | spx | 2.86e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| GFBIJKNM_22 | 35214 | 34972 | SpoVG | 7.67e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| GFBIJKNM_26 | 5975 | 6172 | CspA | 3.67e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| GFBIJKNM_27 | 86614 | 86420 | CspD | 3.30e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| GFBIJKNM_27 | 86614 | 86417 | CspB | 6.85e-28 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| GFBIJKNM_77 | 6677 | 6870 | Col(SD853) | 194 / 194 | 97.94 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GFBIJKNM_1 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_2 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_3 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_4 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_5 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_11 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_13 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_14 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_15 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_16 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_17 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_18 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_19 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_20 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_21 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_22 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_23 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_24 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_25 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_26 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_27 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_28 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_29 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_30 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_31 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_32 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_33 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_34 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_35 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_36 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_37 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_38 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_39 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_40 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_41 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_42 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_43 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_44 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_45 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_46 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_47 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_48 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_49 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_50 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_51 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_53 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_54 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_55 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_56 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_57 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_58 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_59 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_61 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_62 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_68 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_70 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_73 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_74 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_75 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_76 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_77 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_78 | 0.0 | 1.0 | 0.0 |
| GFBIJKNM_79 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_80 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_81 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_82 | 1.0 | 0.0 | 1.0 |
| GFBIJKNM_84 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GFBIJKNM_20 | 115542 | 142240 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| GFBIJKNM_32 | 1658 | 17245 | False | Myoviridae | VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG0906, VOG7718, VOG3390, VOG7895, VOG0753 |
| GFBIJKNM_50 | 73446 | 97080 | False | Siphoviridae | VOG2703, VOG4841, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4626, VOG3390, VOG4705, VOG4612, VOG3390, VOG7896, VOG5221 |
Phigaro v2.4.0 (Database: Jan. 2024 release)