Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 8 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AHBBDOLG_2 | 836089 | 836427 | ykkC | ARO:3003063 | ykkC | 97.32 | 100.00 | AL009126.1 |
| AHBBDOLG_2 | 835772 | 836089 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| AHBBDOLG_4 | 350215 | 351648 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| AHBBDOLG_4 | 303254 | 303847 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| AHBBDOLG_6 | 221651 | 224221 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| AHBBDOLG_5 | 220387 | 221241 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| AHBBDOLG_5 | 201917 | 203119 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| AHBBDOLG_4 | 61411 | 63057 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| AHBBDOLG_3 | 192460 | 193836 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.13 | 100.00 | D12567 |
| AHBBDOLG_5 | 220387 | 221241 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| AHBBDOLG_4 | 363541 | 364460 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AHBBDOLG_3 | 192460 | 193833 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_063856081.1 |
| AHBBDOLG_5 | 220390 | 221241 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| AHBBDOLG_4 | 61411 | 63054 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| AHBBDOLG_3 | 195839 | 196357 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AHBBDOLG_1 | 203314 | 204495 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| AHBBDOLG_1 | 204978 | 206120 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| AHBBDOLG_1 | 683274 | 684212 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| AHBBDOLG_1 | 204510 | 204959 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| AHBBDOLG_1 | 679591 | 680376 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.11 | 100.00 | NP_391080 |
| AHBBDOLG_2 | 301331 | 303094 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| AHBBDOLG_2 | 181746 | 182387 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| AHBBDOLG_1 | 680402 | 681598 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.66 | 100.00 | NP_391079 |
| AHBBDOLG_1 | 684232 | 691368 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.66 | 100.00 | NP_391076 |
| AHBBDOLG_1 | 681627 | 683246 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.04 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AHBBDOLG_3 | 33416 | 32280 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| AHBBDOLG_3 | 148690 | 147821 | pdxS | 3.59e-150 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| AHBBDOLG_2 | 525849 | 525073 | CodY | 7.46e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| AHBBDOLG_1 | 356023 | 355454 | ClpP | 6.70e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| AHBBDOLG_7 | 112463 | 112855 | spxA1 | 1.25e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| AHBBDOLG_7 | 112463 | 112855 | spx | 7.45e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| AHBBDOLG_3 | 110516 | 110274 | SpoVG | 6.59e-38 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| AHBBDOLG_4 | 107558 | 107361 | CspA | 1.72e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| AHBBDOLG_6 | 311275 | 311081 | CspB | 3.09e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| AHBBDOLG_12 | 6995 | 7188 | Col(SD853) | 194 / 194 | 97.42 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AHBBDOLG_1 | 0.0 | 1.0 | 0.0 |
| AHBBDOLG_2 | 0.0 | 1.0 | 0.0 |
| AHBBDOLG_3 | 0.0 | 1.0 | 0.0 |
| AHBBDOLG_4 | 0.0 | 1.0 | 0.0 |
| AHBBDOLG_5 | 0.0 | 1.0 | 0.0 |
| AHBBDOLG_6 | 0.0 | 1.0 | 0.0 |
| AHBBDOLG_7 | 0.0 | 1.0 | 0.0 |
| AHBBDOLG_8 | 0.0 | 1.0 | 0.0 |
| AHBBDOLG_9 | 0.0 | 1.0 | 0.0 |
| AHBBDOLG_10 | 0.0 | 1.0 | 0.0 |
| AHBBDOLG_11 | 0.0 | 1.0 | 0.0 |
| AHBBDOLG_12 | 1.0 | 0.0 | 1.0 |
| AHBBDOLG_13 | 1.0 | 0.0 | 1.0 |
| AHBBDOLG_14 | 0.0 | 1.0 | 0.0 |
| AHBBDOLG_15 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AHBBDOLG_1 | 460767 | 500748 | True | Siphoviridae | VOG3490, VOG0275, VOG0275, VOG1563, VOG9667, VOG9667, VOG7581, VOG4566, VOG0189, VOG6587, VOG1336, VOG0245, VOG5836, VOG0397, VOG0102, VOG0044, VOG0198, VOG0275, VOG4841, VOG0713, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG9583, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG4358, VOG4612, VOG6243, VOG4705, VOG0641 |
| AHBBDOLG_2 | 865891 | 894090 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667, VOG4602 |
| AHBBDOLG_4 | 8217 | 17429 | False | Siphoviridae | VOG0186, VOG8233, VOG4603, VOG4552, VOG0650, VOG9667, VOG9667, VOG4602, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)