Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 21 | 33 | 39.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BCDHDMIP_8 | 83036 | 83887 | rfbD | 0.0 | Capsule | Immune modulation | 94.37 | 100.00 | WP_014621820 |
| BCDHDMIP_15 | 88602 | 90062 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 93.84 | 100.14 | WP_011226049 |
| BCDHDMIP_15 | 88602 | 90062 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.78 | 99.93 | WP_011681177 |
| BCDHDMIP_8 | 80039 | 80848 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 93.71 | 99.88 | WP_002948020 |
| BCDHDMIP_15 | 92303 | 93670 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.13 | 100.00 | WP_011226045 |
| BCDHDMIP_15 | 92303 | 93668 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 92.97 | 99.85 | WP_011681173 |
| BCDHDMIP_15 | 88602 | 90062 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 92.95 | 100.14 | WP_041827043 |
| BCDHDMIP_8 | 76015 | 77771 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.43 | 98.43 | WP_011226325 |
| BCDHDMIP_8 | 78834 | 80039 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.37 | 100.00 | WP_011226326 |
| BCDHDMIP_13 | 341214 | 341807 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 92.09 | 100.00 | WP_011226149 |
| BCDHDMIP_8 | 81785 | 82933 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 92.00 | 99.91 | WP_041828290 |
| BCDHDMIP_8 | 80832 | 81788 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 91.95 | 100.00 | WP_011226328 |
| BCDHDMIP_15 | 90063 | 90794 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.94 | 100.00 | WP_011226048 |
| BCDHDMIP_13 | 341214 | 341807 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 91.92 | 100.00 | WP_011226149 |
| BCDHDMIP_13 | 341214 | 341807 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 91.92 | 100.00 | WP_011226149 |
| BCDHDMIP_8 | 77768 | 78814 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.48 | 99.34 | WP_002948022 |
| BCDHDMIP_13 | 340345 | 341214 | rfbA | 0.0 | Capsule | Immune modulation | 91.26 | 100.00 | WP_011681286 |
| BCDHDMIP_15 | 90063 | 90794 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011681176 |
| BCDHDMIP_15 | 90063 | 90794 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011227248 |
| BCDHDMIP_15 | 91505 | 92245 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 90.84 | 99.87 | WP_011681174 |
| BCDHDMIP_15 | 209218 | 210876 | fbp54 | 0.0 | FBPs | Adherence | 90.72 | 99.94 | WP_011227179 |
| BCDHDMIP_15 | 91505 | 92245 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 89.89 | 99.87 | WP_011226046 |
| BCDHDMIP_15 | 91505 | 92240 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 89.55 | 98.00 | WP_011227246 |
| BCDHDMIP_12 | 294981 | 295886 | cbpD | 0.0 | Autolysin | Exoenzyme | 89.07 | 93.50 | WP_082302295 |
| BCDHDMIP_8 | 386175 | 387185 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.45 | 99.80 | WP_002986042 |
| BCDHDMIP_13 | 166246 | 167549 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| BCDHDMIP_15 | 90803 | 91495 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 85.45 | 99.86 | WP_011226047 |
| BCDHDMIP_15 | 90803 | 91495 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 85.16 | 99.86 | WP_011227247 |
| BCDHDMIP_15 | 90803 | 91495 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 84.87 | 99.86 | WP_011681175 |
| BCDHDMIP_15 | 371479 | 372240 | srtA | 0.0 | Sortase A | Exoenzyme | 84.29 | 99.74 | - |
| BCDHDMIP_13 | 342010 | 343056 | rfbB | 0.0 | Capsule | Immune modulation | 81.22 | 99.90 | WP_009659423 |
| BCDHDMIP_12 | 6871 | 8154 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.76 | 99.61 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BCDHDMIP_15 | 363344 | 362271 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| BCDHDMIP_15 | 374928 | 375902 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 95.69 | 99.09 | AAK75326 |
| BCDHDMIP_8 | 264190 | 266496 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| BCDHDMIP_15 | 539079 | 540560 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| BCDHDMIP_15 | 344289 | 342043 | metE | 0.0 | methionine biosynthesis enzymes | 93.06 | 100.00 | AAK74738 |
| BCDHDMIP_13 | 73260 | 74228 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| BCDHDMIP_1 | 24540 | 25511 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| BCDHDMIP_13 | 314961 | 316691 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| BCDHDMIP_8 | 124300 | 123149 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| BCDHDMIP_8 | 95818 | 93830 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| BCDHDMIP_8 | 586029 | 584551 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| BCDHDMIP_13 | 166246 | 167547 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| BCDHDMIP_8 | 570673 | 568781 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| BCDHDMIP_15 | 339120 | 340829 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| BCDHDMIP_1 | 60577 | 61668 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| BCDHDMIP_15 | 182838 | 184199 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| BCDHDMIP_8 | 435220 | 436230 | pdh | 0.0 | pyruvate dehydrogenase | 82.49 | 99.70 | ABP91006 |
| BCDHDMIP_13 | 151840 | 152730 | bglB | 5.29e-178 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| BCDHDMIP_15 | 298290 | 299240 | SP_0676 | 7.56e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| BCDHDMIP_15 | 341852 | 340995 | metF | 9.14e-170 | methionine biosynthesis enzymes | 88.11 | 99.31 | AAK74739 |
| BCDHDMIP_15 | 476964 | 476293 | rr01 | 1.17e-126 | response regulator | 84.82 | 99.56 | CAB54566 |
| BCDHDMIP_15 | 42923 | 43627 | covR | 2.72e-126 | responder protein | 85.53 | 100.00 | AAK71319 |
| BCDHDMIP_15 | 335151 | 334480 | ciaR | 5.85e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| BCDHDMIP_15 | 335151 | 334483 | CiaR | 1.08e-124 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| BCDHDMIP_15 | 42923 | 43627 | walR | 6.18e-120 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| BCDHDMIP_12 | 146048 | 146734 | CovR | 7.04e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| BCDHDMIP_1 | 61841 | 62236 | SpxA2 | 1.29e-75 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| BCDHDMIP_15 | 171369 | 170968 | SpxA1 | 1.25e-69 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| BCDHDMIP_10 | 12152 | 12565 | GrpE | 1.44e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| BCDHDMIP_12 | 169266 | 169553 | spr0479 | 8.52e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| BCDHDMIP_13 | 31905 | 32159 | spr1327 | 1.94e-44 | essential gene | 92.94 | 100.00 | AAL00131 |
| BCDHDMIP_8 | 527579 | 527842 | spr0175 | 3.09e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| BCDHDMIP_8 | 528403 | 528669 | spr0177 | 6.85e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| BCDHDMIP_12 | 8321 | 8812 | RopE (M5005_Spy_1611) | 1.56e-72 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| BCDHDMIP_8 | 264190 | 266490 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.36 | 98.08 | MYN69560 |
| BCDHDMIP_8 | 471649 | 470630 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BCDHDMIP_1 | 0.0 | 1.0 | 0.0 |
| BCDHDMIP_2 | 0.0 | 1.0 | 0.0 |
| BCDHDMIP_5 | 0.0 | 1.0 | 0.0 |
| BCDHDMIP_6 | 1.0 | 0.0 | 1.0 |
| BCDHDMIP_7 | 1.0 | 0.0 | 1.0 |
| BCDHDMIP_8 | 0.0 | 1.0 | 0.0 |
| BCDHDMIP_9 | 0.0 | 1.0 | 0.0 |
| BCDHDMIP_10 | 0.0 | 1.0 | 0.0 |
| BCDHDMIP_12 | 0.0 | 1.0 | 0.0 |
| BCDHDMIP_13 | 0.0 | 1.0 | 0.0 |
| BCDHDMIP_14 | 0.0 | 1.0 | 0.0 |
| BCDHDMIP_15 | 0.0 | 1.0 | 0.0 |
| BCDHDMIP_16 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)