Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 7 | 16.43 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCBPLBCI_11 | 633602 | 634456 | aadK | ARO:3002627 | aadK | 99.30 | 100.00 | AL009126.1 |
| MCBPLBCI_22 | 518803 | 519141 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MCBPLBCI_22 | 518486 | 518803 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MCBPLBCI_11 | 386609 | 387778 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MCBPLBCI_46 | 205305 | 206951 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| MCBPLBCI_47 | 109695 | 110615 | mphK | ARO:3004541 | mphK | 97.69 | 100.00 | CP010314.1 |
| MCBPLBCI_47 | 96360 | 97799 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| MCBPLBCI_47 | 46647 | 47240 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| MCBPLBCI_1 | 25861 | 28431 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MCBPLBCI_11 | 633602 | 634456 | aadK | Streptomycin | 99.18 | 100.00 | M26879 |
| MCBPLBCI_47 | 109695 | 110615 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCBPLBCI_11 | 633605 | 634456 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 99.30 | 100.00 | WP_003229862.1 |
| MCBPLBCI_46 | 205308 | 206951 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| MCBPLBCI_47 | 109707 | 110615 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.69 | 99.02 | WP_003246254.1 |
| MCBPLBCI_33 | 40925 | 41443 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| MCBPLBCI_11 | 58747 | 61344 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.71 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MCBPLBCI_44 | 377678 | 378463 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.87 | 100.00 | NP_391080 |
| MCBPLBCI_11 | 172588 | 173230 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| MCBPLBCI_33 | 533791 | 534972 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| MCBPLBCI_44 | 381361 | 382299 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| MCBPLBCI_11 | 9420 | 11183 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| MCBPLBCI_44 | 378489 | 379685 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| MCBPLBCI_33 | 534987 | 535436 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| MCBPLBCI_33 | 535455 | 536597 | capA | 0.0 | Capsule | Immune modulation | 98.86 | 100.00 | NP_391469 |
| MCBPLBCI_44 | 382319 | 389455 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.84 | 100.00 | NP_391076 |
| MCBPLBCI_44 | 379714 | 381333 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.16 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MCBPLBCI_50 | 28030 | 26894 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MCBPLBCI_3 | 4649 | 5518 | pdxS | 1.14e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MCBPLBCI_22 | 209904 | 209128 | CodY | 7.83e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MCBPLBCI_44 | 103677 | 103108 | ClpP | 2.93e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MCBPLBCI_22 | 687848 | 687456 | spxA1 | 6.15e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MCBPLBCI_22 | 687848 | 687456 | spx | 3.67e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| MCBPLBCI_2 | 20865 | 21107 | SpoVG | 7.55e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MCBPLBCI_46 | 159898 | 160095 | CspA | 1.02e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MCBPLBCI_1 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_2 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_3 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_4 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_5 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_6 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_7 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_11 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_22 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_33 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_44 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_46 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_47 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_48 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_49 | 0.0 | 1.0 | 0.0 |
| MCBPLBCI_50 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MCBPLBCI_11 | 564993 | 587695 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG4773, VOG10227 |
| MCBPLBCI_22 | 548997 | 575521 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490 |
Phigaro v2.4.0 (Database: Jan. 2024 release)