Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 9 | 10 | 2 | 13.60 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BPNKCPDM_1 | 2837454 | 2838275 | aadK | ARO:3002627 | aadK | 98.17 | 96.13 | AL009126.1 |
| BPNKCPDM_1 | 2818845 | 2820047 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| BPNKCPDM_1 | 1459541 | 1459858 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BPNKCPDM_1 | 397365 | 397958 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| BPNKCPDM_1 | 346847 | 348280 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| BPNKCPDM_1 | 334042 | 334962 | mphK | ARO:3004541 | mphK | 99.35 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BPNKCPDM_1 | 334042 | 334962 | mph(K) | Spiramycin, Telithromycin | 99.24 | 100.00 | NC_000964 |
| BPNKCPDM_1 | 2837456 | 2838307 | aadK | Streptomycin | 99.18 | 99.65 | M26879 |
| BPNKCPDM_1 | 4297565 | 4298939 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.91 | 99.85 | D12567 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BPNKCPDM_1 | 334042 | 334959 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.35 | 100.00 | WP_003246254.1 |
| BPNKCPDM_1 | 2837457 | 2838278 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.54 | 96.48 | WP_003229862.1 |
| BPNKCPDM_1 | 4295085 | 4295603 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| BPNKCPDM_1 | 2266406 | 2268913 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.86 | 96.54 | WP_087347987.1 |
| BPNKCPDM_1 | 641112 | 642104 | vmlR | ABC-F type ribosomal protection protein VmlR | PARTIALX | 97.89 | 60.51 | WP_003234144.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BPNKCPDM_1 | 3389090 | 3390028 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391077 |
| BPNKCPDM_1 | 3390056 | 3391675 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391078 |
| BPNKCPDM_1 | 2376612 | 2377253 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| BPNKCPDM_1 | 2216536 | 2218299 | dep/capD | 0.0 | Capsule | Immune modulation | 99.94 | 100.00 | NP_389723 |
| BPNKCPDM_1 | 3391704 | 3392900 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.67 | 100.00 | NP_391079 |
| BPNKCPDM_1 | 3798347 | 3799527 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 99.92 | NP_391471 |
| BPNKCPDM_1 | 3796725 | 3797865 | capA | 0.0 | Capsule | Immune modulation | 99.47 | 99.83 | NP_391469 |
| BPNKCPDM_1 | 3392926 | 3393711 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| BPNKCPDM_1 | 3797884 | 3798333 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| BPNKCPDM_1 | 3381938 | 3389070 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.86 | 99.94 | NP_391076 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BPNKCPDM_1 | 132226 | 133362 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BPNKCPDM_1 | 3644050 | 3644619 | ClpP | 2.60e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BPNKCPDM_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BPNKCPDM_1 | 166521 | 174264 | False | Siphoviridae | VOG4602, VOG8483, VOG8483, VOG6495, VOG8241, VOG3549, VOG0083, VOG0083, VOG1309 |
| BPNKCPDM_1 | 185646 | 207143 | False | Myoviridae | VOG9315, VOG4773, VOG4688, VOG9975, VOG7676, VOG4685, VOG4685, VOG0749, VOG4589, VOG4870, VOG3890, VOG3890, VOG3891, VOG3892, VOG3636, VOG6163, VOG4865, VOG4865, VOG4885, VOG3894, VOG4691 |
| BPNKCPDM_1 | 704145 | 722939 | False | Siphoviridae | VOG6005, VOG0796, VOG10227, VOG0720, VOG4564, VOG7363, VOG4555, VOG1329, VOG3480, VOG0816, VOG0724, VOG0799, VOG2621, VOG0725, VOG1323, VOG9745, VOG4605, VOG4599 |
| BPNKCPDM_1 | 1403298 | 1428053 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG9315, VOG10227, VOG4773, VOG4773, VOG4773, VOG4685, VOG0749, VOG3890, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612 |
| BPNKCPDM_1 | 1964771 | 1985053 | False | Siphoviridae | VOG0054, VOG8537, VOG4574, VOG7900, VOG7900, VOG7900, VOG9712, VOG5042, VOG5042, VOG3389, VOG4910, VOG6163, VOG9312, VOG4662 |
| BPNKCPDM_1 | 2040001 | 2047441 | False | Siphoviridae | VOG0322, VOG7476, VOG0025, VOG0025, VOG4551, VOG0918, VOG0918, VOG0918, VOG1198, VOG1198, VOG1198 |
| BPNKCPDM_1 | 2773712 | 2800882 | False | Myoviridae | VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG3890, VOG3890, VOG4870, VOG4589, VOG0749, VOG6947, VOG4685, VOG4685, VOG4688, VOG4773, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060 |
Phigaro v2.4.0 (Database: Jan. 2024 release)