Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 2 | 7 | 7.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BFPHJGEJ_6 | 159455 | 160504 | clbA | ARO:3002814 | clbA | 99.43 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BFPHJGEJ_6 | 159455 | 160490 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.80 | 98.67 | KR610408 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BFPHJGEJ_6 | 159455 | 160501 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 99.43 | 100.00 | WP_012116915.1 |
| BFPHJGEJ_1 | 134506 | 137100 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.11 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BFPHJGEJ_3 | 517969 | 519150 | capB | 0.0 | Capsule | Immune modulation | 82.04 | 99.66 | NP_391471 |
| BFPHJGEJ_3 | 519165 | 519614 | capC | 2.03e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BFPHJGEJ_13 | 28131 | 26995 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| BFPHJGEJ_16 | 12465 | 11596 | pdxS | 1.36e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| BFPHJGEJ_1 | 336016 | 335240 | CodY | 1.15e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BFPHJGEJ_1 | 837445 | 837053 | spxA1 | 2.10e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| BFPHJGEJ_6 | 141603 | 141800 | CspA | 1.31e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BFPHJGEJ_5 | 143786 | 143980 | CspD | 6.32e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| BFPHJGEJ_5 | 143786 | 143983 | CspB | 1.31e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| BFPHJGEJ_6 | 141603 | 141797 | CspR | 1.71e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| BFPHJGEJ_4 | 150794 | 151666 | lipA (SAUSA300_0829) | 9.40e-163 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BFPHJGEJ_1 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_2 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_3 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_4 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_5 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_6 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_7 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_8 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_9 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_10 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_11 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_12 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_13 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_14 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_15 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_16 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_17 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_18 | 0.0 | 1.0 | 0.0 |
| BFPHJGEJ_21 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BFPHJGEJ_1 | 715205 | 741179 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
Phigaro v2.4.0 (Database: Jan. 2024 release)