safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
5	0	0	5.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
GBFICMBK_1	277883	279088	Mef(En2)	ARO:3004659	Mef(En2)	99.25	100.00	AF251288.1
GBFICMBK_12	217946	218836	CblA-1	ARO:3002999	CblA-1	98.99	100.00	GQ343019.1
GBFICMBK_23	79959	80759	ErmF	ARO:3000498	ErmF	98.87	100.00	M17124.1
GBFICMBK_33	1875	3833	tet(Q)	ARO:3000191	tet(Q)	100.00	99.39	Z21523.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
GBFICMBK_33	1923	3835	tet(Q)	Doxycycline, Tetracycline, Minocycline	100.00	99.33	Z21523
GBFICMBK_23	79959	80759	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.75	100.00	M17808

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
GBFICMBK_1	277349	277858	lnu(AN2)	lincosamide nucleotidyltransferase Lnu(AN2)	EXACTX	100.00	100.00	WP_004308783.1
GBFICMBK_33	1923	3833	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	100.00	99.38	WP_002560998.1
GBFICMBK_23	79959	80756	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	BLASTX	99.25	100.00	WP_002682030.1
GBFICMBK_1	277886	279088	mef(En2)	macrolide efflux MFS transporter Mef(En2)	BLASTX	99.25	100.00	WP_063853729.1
GBFICMBK_12	217949	218836	cblA	CblA family class A beta-lactamase	BLASTX	98.99	100.00	WP_005827792.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
GBFICMBK_1	0.0	1.0	0.0
GBFICMBK_2	0.0	1.0	0.0
GBFICMBK_3	0.0	1.0	0.0
GBFICMBK_4	0.0	1.0	0.0
GBFICMBK_5	0.0	1.0	0.0
GBFICMBK_6	0.0	1.0	0.0
GBFICMBK_7	0.0	1.0	0.0
GBFICMBK_8	0.0	1.0	0.0
GBFICMBK_9	0.0	1.0	0.0
GBFICMBK_10	0.0	1.0	0.0
GBFICMBK_11	0.0	1.0	0.0
GBFICMBK_12	0.0	1.0	0.0
GBFICMBK_13	0.0	1.0	0.0
GBFICMBK_14	0.0	1.0	0.0
GBFICMBK_15	1.0	0.0	1.0
GBFICMBK_16	0.0	1.0	0.0
GBFICMBK_17	0.0	1.0	0.0
GBFICMBK_18	0.0	1.0	0.0
GBFICMBK_19	0.0	1.0	0.0
GBFICMBK_20	0.0	1.0	0.0
GBFICMBK_21	1.0	0.0	1.0
GBFICMBK_22	1.0	0.0	1.0
GBFICMBK_23	0.0	1.0	0.0
GBFICMBK_24	0.0	1.0	0.0
GBFICMBK_25	1.0	0.0	1.0
GBFICMBK_26	0.0	1.0	0.0
GBFICMBK_27	1.0	0.0	1.0
GBFICMBK_28	1.0	0.0	1.0
GBFICMBK_29	1.0	0.0	1.0
GBFICMBK_30	0.0	1.0	0.0
GBFICMBK_32	1.0	0.0	1.0
GBFICMBK_33	1.0	0.0	1.0
GBFICMBK_34	0.0	1.0	0.0
GBFICMBK_35	1.0	0.0	1.0
GBFICMBK_36	1.0	0.0	1.0
GBFICMBK_37	1.0	0.0	1.0
GBFICMBK_38	1.0	0.0	1.0
GBFICMBK_39	1.0	0.0	1.0
GBFICMBK_42	0.0	1.0	0.0
GBFICMBK_43	0.0	1.0	0.0
GBFICMBK_44	0.0	1.0	0.0
GBFICMBK_45	0.0	1.0	0.0
GBFICMBK_46	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
GBFICMBK_1	17353	25766	False	Siphoviridae	VOG0749,
GBFICMBK_1	402914	413101	False	Unknown	VOG3992,
GBFICMBK_42	78197	90427	False	Siphoviridae	VOG4690,

Phigaro v2.4.0 (Database: Jan. 2024 release)