Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 16 | 33 | 36.73 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FNBGPOIG_54 | 52082 | 54001 | tet(M) | ARO:3000186 | tet(M) | 98.90 | 100.00 | AB039845.1 |
| FNBGPOIG_54 | 50238 | 50975 | ErmB | ARO:3000375 | ErmB | 97.96 | 98.79 | AF242872.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FNBGPOIG_54 | 50238 | 50975 | erm(B) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | U86375 |
| FNBGPOIG_54 | 52082 | 54001 | tet(M) | Doxycycline, Tetracycline, Minocycline | 99.64 | 100.00 | X92947 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FNBGPOIG_54 | 50241 | 50975 | erm(B) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | EXACTX | 100.00 | 100.00 | WP_001038790.1 |
| FNBGPOIG_54 | 52085 | 54001 | tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | EXACTX | 100.00 | 100.00 | WP_000691736.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FNBGPOIG_53 | 84671 | 85387 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 94.42 | 100.00 | WP_011681442 |
| FNBGPOIG_1 | 6221 | 6952 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 94.40 | 100.00 | WP_011227248 |
| FNBGPOIG_53 | 88112 | 88963 | rfbD | 0.0 | Capsule | Immune modulation | 94.25 | 100.00 | WP_014621820 |
| FNBGPOIG_1 | 6221 | 6952 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.72 | 100.00 | WP_011226048 |
| FNBGPOIG_1 | 6221 | 6952 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.44 | 100.00 | WP_011681176 |
| FNBGPOIG_53 | 83555 | 84325 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 92.35 | 99.23 | WP_011681440 |
| FNBGPOIG_1 | 4760 | 6220 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 91.26 | 99.86 | WP_011681177 |
| FNBGPOIG_12 | 151938 | 152531 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| FNBGPOIG_12 | 151938 | 152531 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| FNBGPOIG_12 | 151938 | 152531 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| FNBGPOIG_1 | 4760 | 6220 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 90.65 | 100.00 | WP_011226049 |
| FNBGPOIG_1 | 107487 | 109144 | fbp54 | 0.0 | FBPs | Adherence | 90.30 | 99.88 | WP_011227179 |
| FNBGPOIG_1 | 4760 | 6220 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 89.97 | 100.00 | WP_041827043 |
| FNBGPOIG_17 | 10175 | 11080 | cbpD | 0.0 | Autolysin | Exoenzyme | 89.96 | 93.50 | WP_082302295 |
| FNBGPOIG_1 | 6961 | 7653 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011226047 |
| FNBGPOIG_1 | 6961 | 7653 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011227247 |
| FNBGPOIG_1 | 6961 | 7653 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| FNBGPOIG_4 | 28552 | 29855 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| FNBGPOIG_13 | 15945 | 16958 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 85.67 | 99.90 | WP_002986042 |
| FNBGPOIG_1 | 254736 | 255497 | srtA | 0.0 | Sortase A | Exoenzyme | 84.40 | 99.87 | - |
| FNBGPOIG_12 | 152801 | 153847 | rfbB | 0.0 | Capsule | Immune modulation | 83.49 | 99.90 | WP_002263087 |
| FNBGPOIG_16 | 1050 | 2333 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.76 | 99.61 | WP_000107753 |
| FNBGPOIG_12 | 151069 | 151938 | rfbA | 0.0 | Capsule | Immune modulation | 80.28 | 99.77 | WP_002904719 |
| FNBGPOIG_1 | 7663 | 8333 | STU_RS14635 | 4.59e-149 | Capsule | Immune modulation | 81.13 | 90.28 | WP_011226046 |
| FNBGPOIG_1 | 7663 | 8333 | STER_RS05305 | 2.14e-147 | Capsule | Immune modulation | 80.98 | 90.28 | WP_011681174 |
| FNBGPOIG_1 | 7663 | 8333 | STR_RS05250 | 2.17e-137 | Capsule | Immune modulation | 80.09 | 89.20 | WP_011227246 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FNBGPOIG_1 | 258186 | 259169 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| FNBGPOIG_1 | 246598 | 245525 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| FNBGPOIG_34 | 12577 | 10271 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.41 | 99.22 | AAK74619 |
| FNBGPOIG_2 | 81909 | 80428 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| FNBGPOIG_45 | 48669 | 49637 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| FNBGPOIG_1 | 227539 | 225293 | metE | 0.0 | methionine biosynthesis enzymes | 92.66 | 100.00 | AAK74738 |
| FNBGPOIG_9 | 5308 | 6279 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.36 | 100.00 | ABF36968 |
| FNBGPOIG_12 | 109528 | 111258 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| FNBGPOIG_53 | 104096 | 102108 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.74 | 100.00 | AE014259_2 |
| FNBGPOIG_8 | 30665 | 31816 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| FNBGPOIG_10 | 7732 | 9210 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| FNBGPOIG_4 | 28552 | 29853 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| FNBGPOIG_10 | 23082 | 24974 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| FNBGPOIG_1 | 222375 | 224084 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| FNBGPOIG_9 | 39746 | 40837 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| FNBGPOIG_55 | 19297 | 20307 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| FNBGPOIG_1 | 93722 | 95083 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| FNBGPOIG_4 | 14146 | 15036 | bglB | 8.98e-179 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| FNBGPOIG_1 | 182406 | 183356 | SP_0676 | 5.33e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| FNBGPOIG_1 | 225103 | 224246 | metF | 1.57e-168 | methionine biosynthesis enzymes | 87.06 | 99.31 | AAK74739 |
| FNBGPOIG_15 | 2372 | 3076 | covR | 1.33e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| FNBGPOIG_1 | 326819 | 326148 | rr01 | 7.76e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| FNBGPOIG_1 | 217710 | 217039 | ciaR | 3.46e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| FNBGPOIG_1 | 217710 | 217042 | CiaR | 6.40e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| FNBGPOIG_15 | 2372 | 3076 | walR | 3.01e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| FNBGPOIG_23 | 134544 | 135230 | CovR | 1.43e-120 | serves to repress GAS virulence factor-encoding genes | 82.10 | 100.00 | ACE75886 |
| FNBGPOIG_9 | 41010 | 41405 | SpxA2 | 4.08e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| FNBGPOIG_1 | 82254 | 81853 | SpxA1 | 2.30e-69 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| FNBGPOIG_5 | 11793 | 11380 | GrpE | 5.16e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| FNBGPOIG_23 | 157750 | 158037 | spr0479 | 4.52e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| FNBGPOIG_45 | 17163 | 17417 | spr1327 | 3.48e-46 | essential gene | 94.12 | 100.00 | AAL00131 |
| FNBGPOIG_7 | 13880 | 14143 | spr0175 | 2.80e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| FNBGPOIG_7 | 14705 | 14971 | spr0177 | 6.22e-42 | essential gene | 83.15 | 88.12 | AAK98981 |
| FNBGPOIG_16 | 2500 | 2991 | RopE (M5005_Spy_1611) | 1.35e-73 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| FNBGPOIG_34 | 12577 | 10277 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| FNBGPOIG_55 | 55437 | 54418 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| FNBGPOIG_54 | 62278 | 63483 | repUS43 | 1206 / 1206 | 100.00 | CP003584 |
| FNBGPOIG_30 | 1750 | 2330 | repUS2 | 584 / 681 | 80.48 | BFU30316 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FNBGPOIG_1 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_2 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_3 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_4 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_5 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_6 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_7 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_8 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_9 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_10 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_11 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_12 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_13 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_14 | 1.0 | 0.0 | 1.0 |
| FNBGPOIG_15 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_16 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_17 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_18 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_19 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_20 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_21 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_23 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_25 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_26 | 1.0 | 0.0 | 1.0 |
| FNBGPOIG_29 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_30 | 1.0 | 0.0 | 1.0 |
| FNBGPOIG_31 | 1.0 | 0.0 | 1.0 |
| FNBGPOIG_32 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_33 | 1.0 | 0.0 | 1.0 |
| FNBGPOIG_34 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_35 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_36 | 1.0 | 0.0 | 1.0 |
| FNBGPOIG_37 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_38 | 1.0 | 0.0 | 1.0 |
| FNBGPOIG_45 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_53 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_54 | 1.0 | 0.0 | 1.0 |
| FNBGPOIG_55 | 0.0 | 1.0 | 0.0 |
| FNBGPOIG_56 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FNBGPOIG_12 | 227305 | 265951 | False | Siphoviridae | VOG6127, VOG4771, VOG10169, VOG1564, VOG9469, VOG3478, VOG4555, VOG0651, VOG5996, VOG4566, VOG0189, VOG3430, VOG1309, VOG0685, VOG0226, VOG4693, VOG0655, VOG0656, VOG6140, VOG0152, VOG4567, VOG0657, VOG5439, VOG0658, VOG0198, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209, VOG4545, VOG4605, VOG4599, VOG0647, VOG0648, VOG0641, VOG0054, VOG4649 |
Phigaro v2.4.0 (Database: Jan. 2024 release)