Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 19 | 35 | 39.82 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HHFOALAE_23 | 77916 | 78631 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 96.65 | 99.86 | WP_011681442 |
| HHFOALAE_23 | 81940 | 82791 | rfbD | 0.0 | Capsule | Immune modulation | 95.07 | 100.00 | WP_014621820 |
| HHFOALAE_23 | 78633 | 79601 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 94.94 | 100.00 | WP_011681443 |
| HHFOALAE_29 | 23924 | 25384 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 94.32 | 100.14 | WP_011226049 |
| HHFOALAE_29 | 23924 | 25384 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 94.18 | 100.00 | WP_011681177 |
| HHFOALAE_29 | 23924 | 25384 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.43 | 100.14 | WP_041827043 |
| HHFOALAE_29 | 20318 | 21683 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 93.12 | 99.85 | WP_011681173 |
| HHFOALAE_29 | 20316 | 21683 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 92.98 | 100.00 | WP_011226045 |
| HHFOALAE_23 | 76794 | 77570 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 92.92 | 100.00 | WP_011681440 |
| HHFOALAE_23 | 80568 | 81821 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 92.34 | 99.44 | WP_011681445 |
| HHFOALAE_29 | 23192 | 23923 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.80 | 100.00 | WP_011226048 |
| HHFOALAE_12 | 172347 | 172940 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.75 | 100.00 | WP_011226149 |
| HHFOALAE_12 | 172347 | 172940 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 91.58 | 100.00 | WP_011226149 |
| HHFOALAE_12 | 172347 | 172940 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 91.58 | 100.00 | WP_011226149 |
| HHFOALAE_29 | 23192 | 23923 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.39 | 100.00 | WP_011681176 |
| HHFOALAE_29 | 23192 | 23923 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 91.12 | 100.00 | WP_011227248 |
| HHFOALAE_29 | 21741 | 22481 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 90.84 | 99.87 | WP_011681174 |
| HHFOALAE_28 | 95003 | 96661 | fbp54 | 0.0 | FBPs | Adherence | 90.78 | 99.94 | WP_011227179 |
| HHFOALAE_12 | 171478 | 172347 | rfbA | 0.0 | Capsule | Immune modulation | 90.58 | 100.00 | WP_011681286 |
| HHFOALAE_29 | 21741 | 22481 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 89.89 | 99.87 | WP_011226046 |
| HHFOALAE_29 | 21746 | 22481 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 89.55 | 98.00 | WP_011227246 |
| HHFOALAE_27 | 154445 | 155455 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.45 | 99.80 | WP_002986042 |
| HHFOALAE_1 | 184 | 1487 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| HHFOALAE_1 | 197033 | 197933 | cbpD | 0.0 | Autolysin | Exoenzyme | 88.23 | 92.98 | WP_082302295 |
| HHFOALAE_29 | 22491 | 23183 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 87.18 | 99.86 | WP_011227247 |
| HHFOALAE_29 | 22491 | 23183 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 87.18 | 99.86 | WP_011226047 |
| HHFOALAE_29 | 22491 | 23183 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 86.45 | 99.86 | WP_011681175 |
| HHFOALAE_31 | 70633 | 71394 | srtA | 0.0 | Sortase A | Exoenzyme | 84.67 | 99.87 | - |
| HHFOALAE_12 | 173143 | 174189 | rfbB | 0.0 | Capsule | Immune modulation | 84.16 | 99.90 | WP_002263087 |
| HHFOALAE_14 | 8368 | 9651 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.84 | 99.61 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HHFOALAE_31 | 74083 | 75066 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.04 | 100.00 | AAK75326 |
| HHFOALAE_31 | 62498 | 61425 | MntE | 0.0 | manganese homeostasis | 95.53 | 99.72 | ABF32167 |
| HHFOALAE_27 | 33971 | 36277 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| HHFOALAE_26 | 113094 | 114575 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| HHFOALAE_31 | 43440 | 41194 | metE | 0.0 | methionine biosynthesis enzymes | 93.19 | 100.00 | AAK74738 |
| HHFOALAE_1 | 96730 | 95762 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| HHFOALAE_6 | 4745 | 5716 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.36 | 100.00 | ABF36968 |
| HHFOALAE_12 | 146095 | 147825 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 90.12 | 100.00 | AAK34199 |
| HHFOALAE_23 | 125219 | 124068 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| HHFOALAE_23 | 96694 | 94706 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| HHFOALAE_8 | 25715 | 27193 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| HHFOALAE_1 | 1487 | 186 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| HHFOALAE_1 | 452361 | 450469 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| HHFOALAE_31 | 38272 | 39981 | PGM | 0.0 | phosphoglucomutase | 85.11 | 99.65 | ACH87041 |
| HHFOALAE_6 | 41750 | 42841 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| HHFOALAE_1 | 317756 | 318766 | pdh | 0.0 | pyruvate dehydrogenase | 83.38 | 99.70 | ABP91006 |
| HHFOALAE_28 | 68974 | 70335 | mnmE | 0.0 | central tRNA-modifying GTPase | 83.33 | 99.56 | ABP90420 |
| HHFOALAE_1 | 15896 | 15006 | bglB | 2.67e-177 | putative phospho--glucosidase | 87.54 | 99.66 | AAK33487 |
| HHFOALAE_13 | 11363 | 12313 | SP_0676 | 1.46e-171 | transcription facot | 81.39 | 98.45 | AAK74821 |
| HHFOALAE_31 | 41004 | 40147 | metF | 6.42e-169 | methionine biosynthesis enzymes | 87.06 | 99.31 | AAK74739 |
| HHFOALAE_28 | 121040 | 120255 | pnuC | 1.03e-150 | NAD salvage pathway importer | 87.41 | 97.40 | AAK75931 |
| HHFOALAE_26 | 48638 | 47967 | rr01 | 3.89e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| HHFOALAE_29 | 71068 | 70364 | covR | 5.42e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| HHFOALAE_31 | 34305 | 33634 | ciaR | 8.51e-126 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| HHFOALAE_31 | 34305 | 33637 | CiaR | 1.57e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| HHFOALAE_23 | 123290 | 122523 | potC | 3.70e-124 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 80.08 | 99.61 | AAL00048 |
| HHFOALAE_29 | 71068 | 70364 | walR | 1.23e-120 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| HHFOALAE_4 | 53617 | 52931 | CovR | 1.34e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| HHFOALAE_6 | 43016 | 43411 | SpxA2 | 4.28e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| HHFOALAE_28 | 57506 | 57105 | SpxA1 | 3.20e-70 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| HHFOALAE_5 | 6809 | 6396 | GrpE | 3.74e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| HHFOALAE_4 | 30409 | 30122 | spr0479 | 1.54e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| HHFOALAE_1 | 140553 | 140299 | spr1327 | 2.12e-44 | essential gene | 92.94 | 100.00 | AAL00131 |
| HHFOALAE_1 | 408824 | 409087 | spr0175 | 2.41e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| HHFOALAE_1 | 409648 | 409914 | spr0177 | 5.35e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| HHFOALAE_14 | 8201 | 7710 | RopE (M5005_Spy_1611) | 5.36e-74 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| HHFOALAE_27 | 33971 | 36271 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| HHFOALAE_1 | 354178 | 353159 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HHFOALAE_1 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_2 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_3 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_4 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_5 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_6 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_7 | 1.0 | 0.0 | 1.0 |
| HHFOALAE_8 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_9 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_10 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_11 | 1.0 | 0.0 | 1.0 |
| HHFOALAE_12 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_13 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_14 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_15 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_16 | 1.0 | 0.0 | 1.0 |
| HHFOALAE_17 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_18 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_19 | 1.0 | 0.0 | 1.0 |
| HHFOALAE_21 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_22 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_23 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_24 | 1.0 | 0.0 | 1.0 |
| HHFOALAE_26 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_27 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_28 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_29 | 0.0 | 1.0 | 0.0 |
| HHFOALAE_30 | 1.0 | 0.0 | 1.0 |
| HHFOALAE_31 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)