safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
3	0	0	3.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
MDPFOAEF_48	2981	3871	CblA-1	ARO:3002999	CblA-1	99.32	100.00	GQ343019.1
MDPFOAEF_59	1	1695	tet(Q)	ARO:3000191	tet(Q)	95.57	85.84	Z21523.1
MDPFOAEF_60	1	1695	tet(Q)	ARO:3000191	tet(Q)	95.92	85.84	Z21523.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
MDPFOAEF_60	1	1695	tet(Q)	Doxycycline, Tetracycline, Minocycline	99.82	88.01	L33696

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
MDPFOAEF_60	1	1692	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	PARTIAL_CONTIG_ENDX	99.47	87.99	WP_063856407.1
MDPFOAEF_48	2984	3871	cblA	CblA family class A beta-lactamase	BLASTX	99.32	100.00	WP_005827792.1
MDPFOAEF_59	1	1692	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	PARTIAL_CONTIG_ENDX	98.58	87.99	WP_063856409.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
MDPFOAEF_1	0.0	1.0	0.0
MDPFOAEF_2	0.0	1.0	0.0
MDPFOAEF_3	0.0	1.0	0.0
MDPFOAEF_4	0.0	1.0	0.0
MDPFOAEF_5	0.0	1.0	0.0
MDPFOAEF_6	0.0	1.0	0.0
MDPFOAEF_7	0.0	1.0	0.0
MDPFOAEF_8	0.0	1.0	0.0
MDPFOAEF_9	0.0	1.0	0.0
MDPFOAEF_10	0.0	1.0	0.0
MDPFOAEF_11	0.0	1.0	0.0
MDPFOAEF_12	0.0	1.0	0.0
MDPFOAEF_13	0.0	1.0	0.0
MDPFOAEF_14	0.0	1.0	0.0
MDPFOAEF_15	0.0	1.0	0.0
MDPFOAEF_16	0.0	1.0	0.0
MDPFOAEF_17	0.0	1.0	0.0
MDPFOAEF_18	0.0	1.0	0.0
MDPFOAEF_19	0.0	1.0	0.0
MDPFOAEF_20	0.0	1.0	0.0
MDPFOAEF_21	0.0	1.0	0.0
MDPFOAEF_22	1.0	0.0	1.0
MDPFOAEF_23	0.0	1.0	0.0
MDPFOAEF_24	0.0	1.0	0.0
MDPFOAEF_25	0.0	1.0	0.0
MDPFOAEF_26	0.0	1.0	0.0
MDPFOAEF_27	0.0	1.0	0.0
MDPFOAEF_28	0.0	1.0	0.0
MDPFOAEF_29	0.0	1.0	0.0
MDPFOAEF_30	0.0	1.0	0.0
MDPFOAEF_31	0.0	1.0	0.0
MDPFOAEF_32	0.0	1.0	0.0
MDPFOAEF_33	0.0	1.0	0.0
MDPFOAEF_34	0.0	1.0	0.0
MDPFOAEF_35	0.0	1.0	0.0
MDPFOAEF_36	0.0	1.0	0.0
MDPFOAEF_37	0.0	1.0	0.0
MDPFOAEF_38	0.0	1.0	0.0
MDPFOAEF_39	0.0	1.0	0.0
MDPFOAEF_40	1.0	0.0	1.0
MDPFOAEF_41	0.0	1.0	0.0
MDPFOAEF_42	0.0	1.0	0.0
MDPFOAEF_43	0.0	1.0	0.0
MDPFOAEF_45	0.0	1.0	0.0
MDPFOAEF_46	1.0	0.0	1.0
MDPFOAEF_47	1.0	0.0	1.0
MDPFOAEF_48	0.0	1.0	0.0
MDPFOAEF_49	0.0	1.0	0.0
MDPFOAEF_50	1.0	0.0	1.0
MDPFOAEF_51	1.0	0.0	1.0
MDPFOAEF_52	1.0	0.0	1.0
MDPFOAEF_53	1.0	0.0	1.0
MDPFOAEF_54	0.0	1.0	0.0
MDPFOAEF_55	1.0	0.0	1.0
MDPFOAEF_56	0.0	1.0	0.0
MDPFOAEF_57	1.0	0.0	1.0
MDPFOAEF_58	1.0	0.0	1.0
MDPFOAEF_59	1.0	0.0	1.0
MDPFOAEF_60	1.0	0.0	1.0
MDPFOAEF_61	1.0	0.0	1.0
MDPFOAEF_62	0.0	1.0	0.0
MDPFOAEF_63	0.0	1.0	0.0
MDPFOAEF_64	1.0	0.0	1.0
MDPFOAEF_65	1.0	0.0	1.0
MDPFOAEF_66	0.0	1.0	0.0
MDPFOAEF_67	0.0	1.0	0.0
MDPFOAEF_68	1.0	0.0	1.0
MDPFOAEF_69	1.0	0.0	1.0
MDPFOAEF_78	0.0	1.0	0.0
MDPFOAEF_80	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
MDPFOAEF_3	147637	158293	False	Siphoviridae	VOG2803,
MDPFOAEF_21	6160	13662	False	Siphoviridae	VOG7238,
MDPFOAEF_23	93893	95420	False	Unknown	VOG10050,

Phigaro v2.4.0 (Database: Jan. 2024 release)