Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 0 | 1 | 3.16 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MAFOLNHB_1 | 220756 | 222681 | tet(Q) | ARO:3000191 | tet(Q) | 96.41 | 97.56 | Z21523.1 |
| MAFOLNHB_27 | 47 | 847 | ErmF | ARO:3000498 | ErmF | 98.12 | 100.00 | M17124.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MAFOLNHB_1 | 220756 | 222681 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.84 | 100.00 | L33696 |
| MAFOLNHB_27 | 47 | 847 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.38 | 100.00 | M17808 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MAFOLNHB_1 | 220759 | 222681 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.53 | 100.00 | WP_063856407.1 |
| MAFOLNHB_27 | 1060 | 1899 | estT | macrolide hydrolase EstT | BLASTX | 98.58 | 100.00 | WP_185148407.1 |
| MAFOLNHB_27 | 47 | 844 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | BLASTX | 98.50 | 100.00 | WP_002682030.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MAFOLNHB_2 | 147756 | 146176 | GroEL | 0.0 | folding of newly synthesized proteins, preventing misfolding and aggregation | 86.91 | 96.70 | BAA04222 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| MAFOLNHB_54 | 1830 | 2410 | repUS2 | 584 / 681 | 80.48 | BFU30316 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MAFOLNHB_1 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_2 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_3 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_4 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_5 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_6 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_7 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_8 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_9 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_10 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_11 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_12 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_13 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_14 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_15 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_16 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_17 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_18 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_19 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_20 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_21 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_22 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_23 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_24 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_25 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_26 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_27 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_28 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_29 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_30 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_31 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_32 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_33 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_34 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_35 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_36 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_37 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_38 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_39 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_40 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_41 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_42 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_43 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_44 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_45 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_46 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_47 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_48 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_49 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_50 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_51 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_52 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_53 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_54 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_55 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_56 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_57 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_58 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_59 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_60 | 1.0 | 0.0 | 1.0 |
| MAFOLNHB_67 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_70 | 0.0 | 1.0 | 0.0 |
| MAFOLNHB_71 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MAFOLNHB_1 | 275180 | 282026 | False | Siphoviridae | VOG6495, |
Phigaro v2.4.0 (Database: Jan. 2024 release)