Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ILPBNKDL_1 | 2702775 | 2703629 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| ILPBNKDL_1 | 2683437 | 2684639 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| ILPBNKDL_1 | 2460768 | 2461937 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| ILPBNKDL_1 | 1373220 | 1373537 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| ILPBNKDL_1 | 1372882 | 1373220 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| ILPBNKDL_1 | 902648 | 905218 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| ILPBNKDL_1 | 328381 | 328974 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| ILPBNKDL_1 | 277823 | 279256 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| ILPBNKDL_1 | 265037 | 265957 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ILPBNKDL_1 | 2702777 | 2703629 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| ILPBNKDL_1 | 265037 | 265957 | mph(K) | Spiramycin, Telithromycin | 97.61 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ILPBNKDL_1 | 596788 | 598410 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.34 | 98.90 | WP_003234144.1 |
| ILPBNKDL_1 | 2702778 | 2703629 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| ILPBNKDL_1 | 265037 | 265954 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| ILPBNKDL_1 | 4160638 | 4161150 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.06 | 98.84 | WP_003242546.1 |
| ILPBNKDL_1 | 2035729 | 2038326 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ILPBNKDL_1 | 3252604 | 3253542 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| ILPBNKDL_1 | 3678714 | 3679895 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| ILPBNKDL_1 | 2259909 | 2260550 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| ILPBNKDL_1 | 3253570 | 3255189 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.20 | 100.00 | NP_391078 |
| ILPBNKDL_1 | 3256440 | 3257225 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.11 | 100.00 | NP_391080 |
| ILPBNKDL_1 | 3255218 | 3256414 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| ILPBNKDL_1 | 3677089 | 3678231 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| ILPBNKDL_1 | 3245454 | 3252584 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.92 | 99.99 | NP_391076 |
| ILPBNKDL_1 | 1991225 | 1992988 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| ILPBNKDL_1 | 3678250 | 3678699 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ILPBNKDL_1 | 132736 | 133872 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| ILPBNKDL_1 | 19012 | 19881 | pdxS | 3.11e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| ILPBNKDL_1 | 1685369 | 1686145 | CodY | 3.40e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ILPBNKDL_1 | 3521074 | 3521643 | ClpP | 2.52e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ILPBNKDL_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ILPBNKDL_1 | 1315742 | 1344676 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG5384, VOG4705, VOG0753, VOG0136 |
| ILPBNKDL_1 | 2151344 | 2156896 | False | Unknown | VOG4551, VOG0025, VOG7476, VOG0322, VOG6211 |
| ILPBNKDL_1 | 2629829 | 2662887 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796, VOG4693, VOG0189, VOG11060 |
Phigaro v2.4.0 (Database: Jan. 2024 release)