Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 4 | 15.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KMGCDPBG_1 | 2616543 | 2617397 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
| KMGCDPBG_1 | 2597833 | 2599035 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| KMGCDPBG_1 | 2376854 | 2378023 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| KMGCDPBG_1 | 1283911 | 1284228 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| KMGCDPBG_1 | 1283573 | 1283911 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| KMGCDPBG_1 | 826089 | 828659 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| KMGCDPBG_1 | 538231 | 539874 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| KMGCDPBG_1 | 235797 | 237230 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| KMGCDPBG_1 | 222982 | 223902 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KMGCDPBG_1 | 222982 | 223902 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| KMGCDPBG_1 | 2616543 | 2617397 | aadK | Streptomycin | 98.95 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KMGCDPBG_1 | 222982 | 223899 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| KMGCDPBG_1 | 2616546 | 2617397 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| KMGCDPBG_1 | 538234 | 539874 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| KMGCDPBG_1 | 4082900 | 4083418 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
| KMGCDPBG_1 | 2080633 | 2083230 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.65 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KMGCDPBG_1 | 3177007 | 3177792 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| KMGCDPBG_1 | 3598496 | 3599677 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| KMGCDPBG_1 | 2178822 | 2179463 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| KMGCDPBG_1 | 3598032 | 3598481 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| KMGCDPBG_1 | 3175785 | 3176981 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| KMGCDPBG_1 | 3596871 | 3598013 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| KMGCDPBG_1 | 2031268 | 2033031 | dep/capD | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_389723 |
| KMGCDPBG_1 | 3166015 | 3173151 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.74 | 100.00 | NP_391076 |
| KMGCDPBG_1 | 3173171 | 3174109 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| KMGCDPBG_1 | 3174137 | 3175756 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KMGCDPBG_1 | 102938 | 104074 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| KMGCDPBG_1 | 4391 | 5260 | pdxS | 3.08e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KMGCDPBG_1 | 1595397 | 1596173 | CodY | 3.36e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KMGCDPBG_1 | 3440380 | 3440949 | ClpP | 2.49e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KMGCDPBG_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KMGCDPBG_1 | 1227713 | 1254028 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| KMGCDPBG_1 | 1807777 | 1831680 | False | Siphoviridae | VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG7930, VOG8610, VOG7895, VOG3390, VOG7718, VOG5534, VOG6940, VOG9713, VOG9219, VOG5495, VOG6036, VOG9867 |
| KMGCDPBG_1 | 1873487 | 1882773 | False | Unknown | VOG0101, VOG0048, VOG8921, VOG6211, VOG0322, VOG7476, VOG0025 |
| KMGCDPBG_1 | 2548548 | 2577828 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796, VOG4673, VOG4693, VOG0189, VOG11060, VOG1309 |
Phigaro v2.4.0 (Database: Jan. 2024 release)