Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 3 | 14.46 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ADACGFIB_1 | 3664362 | 3664679 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| ADACGFIB_1 | 3664024 | 3664362 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| ADACGFIB_1 | 3172496 | 3175099 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.28 | 101.29 | AL009126.3 |
| ADACGFIB_1 | 2877674 | 2879320 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
| ADACGFIB_1 | 2608550 | 2609143 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| ADACGFIB_1 | 2557916 | 2559349 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.00 | JYFL01000006.1 |
| ADACGFIB_1 | 2545100 | 2546020 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| ADACGFIB_1 | 663849 | 664703 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| ADACGFIB_1 | 646752 | 647954 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ADACGFIB_1 | 663849 | 664703 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| ADACGFIB_1 | 2545100 | 2546020 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ADACGFIB_1 | 2545100 | 2546008 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| ADACGFIB_1 | 663852 | 664703 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| ADACGFIB_1 | 2877677 | 2879320 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| ADACGFIB_1 | 2242730 | 2243248 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ADACGFIB_1 | 1734517 | 1735698 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| ADACGFIB_1 | 1220169 | 1220954 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| ADACGFIB_1 | 1732892 | 1734034 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| ADACGFIB_1 | 1209177 | 1216313 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| ADACGFIB_1 | 120880 | 122643 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| ADACGFIB_1 | 1216333 | 1217271 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| ADACGFIB_1 | 1218947 | 1220143 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| ADACGFIB_1 | 251785 | 252426 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| ADACGFIB_1 | 1734053 | 1734502 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| ADACGFIB_1 | 1217299 | 1218918 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ADACGFIB_1 | 2293067 | 2293936 | pdxS | 5.36e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| ADACGFIB_1 | 3974772 | 3975548 | CodY | 3.34e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ADACGFIB_1 | 1577140 | 1577709 | ClpP | 2.48e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ADACGFIB_1 | 0.0 | 1.0 | 0.0 |
| ADACGFIB_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ADACGFIB_1 | 1524 | 6808 | False | Siphoviridae | VOG3462, VOG4619, VOG4626, VOG4626, VOG4626, VOG4612, VOG4612, VOG6243, VOG4705, VOG5221 |
| ADACGFIB_1 | 1413952 | 1452479 | True | Siphoviridae | VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG0204, VOG10223, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841, VOG0275, VOG0198, VOG0044, VOG0102, VOG0397, VOG5836, VOG0245, VOG1336, VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
| ADACGFIB_1 | 1542799 | 1553957 | False | Siphoviridae | VOG0602, VOG4574, VOG5221, VOG4705, VOG6243, VOG4612, VOG4626, VOG4619, VOG0801 |
| ADACGFIB_1 | 2179687 | 2188366 | False | Unknown | VOG6422, VOG3239, VOG7443, VOG0944, VOG5532 |
| ADACGFIB_1 | 3605559 | 3634560 | False | Myoviridae | VOG0101, VOG4602, VOG9667, VOG3490, VOG0189, VOG4843, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)