Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PMOKEECP_1 | 3259810 | 3260661 | aadK | ARO:3002627 | aadK | 98.24 | 99.65 | AL009126.1 |
| PMOKEECP_1 | 3241426 | 3242628 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| PMOKEECP_1 | 3057169 | 3058338 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| PMOKEECP_1 | 2105503 | 2105820 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| PMOKEECP_1 | 2105165 | 2105503 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| PMOKEECP_1 | 1594781 | 1597351 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| PMOKEECP_1 | 1253584 | 1255227 | vmlR | ARO:3004476 | vmlR | 98.32 | 100.00 | NC_000964.3 |
| PMOKEECP_1 | 1004405 | 1004998 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| PMOKEECP_1 | 954275 | 955708 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
| PMOKEECP_1 | 939885 | 940805 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| PMOKEECP_1 | 3259810 | 3260661 | aadK | Streptomycin | 98.48 | 99.65 | M26879 |
| PMOKEECP_1 | 939885 | 940805 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PMOKEECP_1 | 939885 | 940793 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| PMOKEECP_1 | 3259813 | 3260661 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| PMOKEECP_1 | 1253587 | 1255227 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.71 | 99.82 | WP_003234144.1 |
| PMOKEECP_1 | 616731 | 617123 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 91.60 | 75.72 | WP_003242546.1 |
| PMOKEECP_1 | 2711850 | 2714447 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| PMOKEECP_1 | 121329 | 122510 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| PMOKEECP_1 | 3818920 | 3819705 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| PMOKEECP_1 | 119704 | 120846 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| PMOKEECP_1 | 2656170 | 2657933 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| PMOKEECP_1 | 3815095 | 3816033 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| PMOKEECP_1 | 2835611 | 2836252 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| PMOKEECP_1 | 120865 | 121314 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| PMOKEECP_1 | 3817698 | 3818894 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391079 |
| PMOKEECP_1 | 3807939 | 3815075 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.56 | 100.00 | NP_391076 |
| PMOKEECP_1 | 3816061 | 3817680 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| PMOKEECP_1 | 780194 | 781330 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| PMOKEECP_1 | 664720 | 665589 | pdxS | 3.12e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| PMOKEECP_1 | 2422114 | 2422890 | CodY | 3.40e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| PMOKEECP_1 | 4166167 | 4166736 | ClpP | 2.52e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| PMOKEECP_3 | 6805 | 7299 | rep21 | 495 / 717 | 90.51 | FR821780 |
| PMOKEECP_3 | 5009 | 5173 | Col(SD853) | 165 / 194 | 86.06 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| PMOKEECP_1 | 0.0 | 1.0 | 0.0 |
| PMOKEECP_2 | 1.0 | 0.0 | 1.0 |
| PMOKEECP_3 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| PMOKEECP_1 | 1298903 | 1308816 | False | Siphoviridae | VOG6513, VOG0275, VOG4602, VOG9667, VOG9667, VOG0650, VOG4552, VOG4603, VOG8233, VOG0186 |
| PMOKEECP_1 | 1310811 | 1336162 | False | Siphoviridae | VOG1985, VOG0198, VOG0713, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG6243, VOG4705, VOG5221, VOG0136 |
| PMOKEECP_1 | 2046700 | 2075701 | False | Myoviridae | VOG0101, VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| PMOKEECP_1 | 3886447 | 3913096 | False | Siphoviridae | VOG3490, VOG4615, VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG2935, VOG4544, VOG4581, VOG4556, VOG4841, VOG0531, VOG0275, VOG0198 |
| PMOKEECP_1 | 4039476 | 4080042 | True | Siphoviridae | VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG0204, VOG10223, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841, VOG0275, VOG0198, VOG0044, VOG0102, VOG0397, VOG5836, VOG0245, VOG1336, VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275, VOG3490 |
Phigaro v2.4.0 (Database: Jan. 2024 release)