Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 7 | 15.78 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HHAPJJKP_1 | 716562 | 717416 | aadK | ARO:3002627 | aadK | 99.30 | 100.00 | AL009126.1 |
| HHAPJJKP_1 | 689215 | 690417 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| HHAPJJKP_1 | 490750 | 491919 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| HHAPJJKP_9 | 428892 | 429209 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| HHAPJJKP_9 | 428554 | 428892 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| HHAPJJKP_14 | 256755 | 259325 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| HHAPJJKP_19 | 108399 | 109319 | mphK | ARO:3004541 | mphK | 96.73 | 100.00 | CP010314.1 |
| HHAPJJKP_19 | 95069 | 96508 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| HHAPJJKP_17 | 29060 | 30706 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HHAPJJKP_1 | 716562 | 717416 | aadK | Streptomycin | 99.18 | 100.00 | M26879 |
| HHAPJJKP_19 | 108399 | 109319 | mph(K) | Spiramycin, Telithromycin | 96.96 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HHAPJJKP_1 | 716565 | 717416 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 99.30 | 100.00 | WP_003229862.1 |
| HHAPJJKP_17 | 29060 | 30703 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| HHAPJJKP_19 | 108402 | 109319 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.73 | 100.00 | WP_003246254.1 |
| HHAPJJKP_1 | 55523 | 58120 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HHAPJJKP_7 | 522453 | 523634 | capB | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | NP_391471 |
| HHAPJJKP_7 | 113201 | 113986 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| HHAPJJKP_1 | 291337 | 291977 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 99.84 | NP_390062 |
| HHAPJJKP_7 | 521989 | 522438 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| HHAPJJKP_7 | 520828 | 521970 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| HHAPJJKP_7 | 111979 | 113175 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391079 |
| HHAPJJKP_1 | 9730 | 11493 | dep/capD | 0.0 | Capsule | Immune modulation | 98.70 | 100.00 | NP_389723 |
| HHAPJJKP_7 | 102209 | 109345 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.68 | 100.00 | NP_391076 |
| HHAPJJKP_7 | 110331 | 111950 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.46 | 100.00 | NP_391078 |
| HHAPJJKP_7 | 109365 | 110303 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.30 | 100.00 | NP_391077 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HHAPJJKP_21 | 28111 | 26975 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| HHAPJJKP_23 | 11305 | 10436 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| HHAPJJKP_9 | 740757 | 741533 | CodY | 8.35e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| HHAPJJKP_7 | 365735 | 366304 | ClpP | 6.30e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| HHAPJJKP_9 | 276378 | 276770 | spxA1 | 6.56e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| HHAPJJKP_9 | 276378 | 276770 | spx | 3.91e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| HHAPJJKP_22 | 34770 | 34528 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| HHAPJJKP_17 | 84526 | 84329 | CspA | 9.96e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HHAPJJKP_1 | 0.0 | 1.0 | 0.0 |
| HHAPJJKP_2 | 0.0 | 1.0 | 0.0 |
| HHAPJJKP_3 | 0.0 | 1.0 | 0.0 |
| HHAPJJKP_4 | 0.0 | 1.0 | 0.0 |
| HHAPJJKP_7 | 0.0 | 1.0 | 0.0 |
| HHAPJJKP_9 | 0.0 | 1.0 | 0.0 |
| HHAPJJKP_14 | 0.0 | 1.0 | 0.0 |
| HHAPJJKP_17 | 0.0 | 1.0 | 0.0 |
| HHAPJJKP_19 | 0.0 | 1.0 | 0.0 |
| HHAPJJKP_21 | 0.0 | 1.0 | 0.0 |
| HHAPJJKP_22 | 0.0 | 1.0 | 0.0 |
| HHAPJJKP_23 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HHAPJJKP_1 | 249007 | 266195 | False | Unknown | VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712 |
| HHAPJJKP_9 | 371115 | 398685 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)