Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 7 | 17.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KFCIKJCE_22 | 659225 | 660394 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| KFCIKJCE_22 | 485926 | 487128 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| KFCIKJCE_22 | 469204 | 470058 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| KFCIKJCE_32 | 426880 | 427197 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| KFCIKJCE_32 | 426542 | 426880 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| KFCIKJCE_40 | 110483 | 111403 | mphK | ARO:3004541 | mphK | 99.01 | 100.00 | CP010314.1 |
| KFCIKJCE_40 | 97156 | 98589 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| KFCIKJCE_40 | 47498 | 48091 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| KFCIKJCE_39 | 29001 | 30644 | vmlR | ARO:3004476 | vmlR | 98.72 | 100.00 | NC_000964.3 |
| KFCIKJCE_38 | 27473 | 30043 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KFCIKJCE_40 | 110483 | 111403 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| KFCIKJCE_22 | 469204 | 470058 | aadK | Streptomycin | 98.48 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KFCIKJCE_40 | 110495 | 111403 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.01 | 99.02 | WP_003246254.1 |
| KFCIKJCE_39 | 29001 | 30641 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.72 | 100.00 | WP_003234144.1 |
| KFCIKJCE_22 | 469204 | 470055 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| KFCIKJCE_11 | 38245 | 38763 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
| KFCIKJCE_22 | 954434 | 957031 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KFCIKJCE_22 | 858797 | 859438 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| KFCIKJCE_11 | 528691 | 529872 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| KFCIKJCE_11 | 951312 | 952097 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| KFCIKJCE_11 | 952123 | 953319 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| KFCIKJCE_11 | 530355 | 531497 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| KFCIKJCE_11 | 954995 | 955933 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| KFCIKJCE_22 | 1009485 | 1011248 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| KFCIKJCE_11 | 529887 | 530336 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| KFCIKJCE_11 | 955953 | 963089 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.88 | 100.00 | NP_391076 |
| KFCIKJCE_11 | 953348 | 954967 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.16 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KFCIKJCE_41 | 31958 | 33094 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| KFCIKJCE_43 | 11333 | 10464 | pdxS | 1.14e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KFCIKJCE_32 | 738364 | 739140 | CodY | 8.27e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KFCIKJCE_11 | 687883 | 687314 | ClpP | 6.40e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| KFCIKJCE_32 | 276792 | 277184 | spxA1 | 6.50e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| KFCIKJCE_32 | 276792 | 277184 | spx | 3.88e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| KFCIKJCE_42 | 20797 | 21039 | SpoVG | 7.55e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| KFCIKJCE_39 | 76618 | 76421 | CspA | 9.39e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KFCIKJCE_1 | 0.0 | 1.0 | 0.0 |
| KFCIKJCE_2 | 0.0 | 1.0 | 0.0 |
| KFCIKJCE_6 | 0.0 | 1.0 | 0.0 |
| KFCIKJCE_11 | 0.0 | 1.0 | 0.0 |
| KFCIKJCE_22 | 0.0 | 1.0 | 0.0 |
| KFCIKJCE_32 | 0.0 | 1.0 | 0.0 |
| KFCIKJCE_38 | 0.0 | 1.0 | 0.0 |
| KFCIKJCE_39 | 0.0 | 1.0 | 0.0 |
| KFCIKJCE_40 | 0.0 | 1.0 | 0.0 |
| KFCIKJCE_41 | 0.0 | 1.0 | 0.0 |
| KFCIKJCE_42 | 0.0 | 1.0 | 0.0 |
| KFCIKJCE_43 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KFCIKJCE_22 | 312744 | 337292 | False | Siphoviridae | VOG4841, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4626, VOG3390, VOG4705, VOG4612, VOG3390, VOG7896, VOG0641, VOG4574 |
| KFCIKJCE_32 | 369943 | 397026 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)