Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BNJJKKCG_1 | 2611265 | 2612119 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| BNJJKKCG_1 | 2592552 | 2593754 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| BNJJKKCG_1 | 2362101 | 2363270 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| BNJJKKCG_1 | 1361762 | 1362079 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BNJJKKCG_1 | 1361424 | 1361762 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BNJJKKCG_1 | 899149 | 901719 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| BNJJKKCG_1 | 588304 | 589947 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
| BNJJKKCG_1 | 337295 | 337888 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| BNJJKKCG_1 | 286765 | 288198 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
| BNJJKKCG_1 | 273952 | 274872 | mphK | ARO:3004541 | mphK | 98.04 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BNJJKKCG_1 | 4104852 | 4106228 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.77 | 100.00 | D12567 |
| BNJJKKCG_1 | 2611267 | 2612119 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| BNJJKKCG_1 | 273952 | 274872 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BNJJKKCG_1 | 4104855 | 4106228 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| BNJJKKCG_1 | 2611268 | 2612119 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| BNJJKKCG_1 | 588307 | 589947 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| BNJJKKCG_1 | 273952 | 274869 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.04 | 100.00 | WP_003246254.1 |
| BNJJKKCG_1 | 4102331 | 4102849 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.33 | 100.00 | WP_003242546.1 |
| BNJJKKCG_1 | 2058422 | 2061019 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BNJJKKCG_1 | 2162945 | 2163586 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| BNJJKKCG_1 | 3623381 | 3624562 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| BNJJKKCG_1 | 3198913 | 3206049 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.54 | 100.00 | NP_391076 |
| BNJJKKCG_1 | 3621756 | 3622898 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| BNJJKKCG_1 | 3209905 | 3210690 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| BNJJKKCG_1 | 3622917 | 3623366 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| BNJJKKCG_1 | 3206069 | 3207007 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| BNJJKKCG_1 | 3208683 | 3209879 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| BNJJKKCG_1 | 2008341 | 2010104 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| BNJJKKCG_1 | 3207035 | 3208654 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.07 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BNJJKKCG_1 | 132399 | 133535 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BNJJKKCG_1 | 18665 | 19534 | pdxS | 3.07e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BNJJKKCG_1 | 1671580 | 1672356 | CodY | 3.36e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BNJJKKCG_1 | 3462027 | 3462596 | ClpP | 2.49e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BNJJKKCG_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BNJJKKCG_1 | 1305126 | 1331939 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
| BNJJKKCG_1 | 2540136 | 2557984 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749 |
| BNJJKKCG_1 | 2744129 | 2767530 | False | Siphoviridae | VOG4615, VOG0136, VOG5221, VOG4705, VOG3390, VOG4612, VOG4705, VOG3390, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886, VOG4581, VOG4841 |
Phigaro v2.4.0 (Database: Jan. 2024 release)