Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OOAFDOEE_1 | 2571936 | 2572790 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| OOAFDOEE_1 | 2554837 | 2556039 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| OOAFDOEE_1 | 2333525 | 2334694 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| OOAFDOEE_1 | 1347673 | 1347990 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| OOAFDOEE_1 | 1347335 | 1347673 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| OOAFDOEE_1 | 884894 | 887464 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| OOAFDOEE_1 | 587209 | 588855 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| OOAFDOEE_1 | 341864 | 342457 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| OOAFDOEE_1 | 275232 | 276665 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| OOAFDOEE_1 | 262419 | 263339 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OOAFDOEE_1 | 262419 | 263339 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| OOAFDOEE_1 | 2571936 | 2572790 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OOAFDOEE_1 | 587212 | 588855 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| OOAFDOEE_1 | 262419 | 263336 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| OOAFDOEE_1 | 2571939 | 2572790 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| OOAFDOEE_1 | 4029251 | 4029769 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 94.22 | 100.00 | WP_003242546.1 |
| OOAFDOEE_1 | 2037829 | 2040426 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.65 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OOAFDOEE_1 | 3552967 | 3554148 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| OOAFDOEE_1 | 3120430 | 3121215 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| OOAFDOEE_1 | 2136006 | 2136647 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| OOAFDOEE_1 | 3119208 | 3120404 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| OOAFDOEE_1 | 1983621 | 1985384 | dep/capD | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | NP_389723 |
| OOAFDOEE_1 | 3552503 | 3552952 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| OOAFDOEE_1 | 3551342 | 3552484 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| OOAFDOEE_1 | 3109438 | 3116574 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.74 | 100.00 | NP_391076 |
| OOAFDOEE_1 | 3116594 | 3117532 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| OOAFDOEE_1 | 3117560 | 3119179 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OOAFDOEE_1 | 132479 | 133615 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| OOAFDOEE_1 | 18666 | 19535 | pdxS | 6.99e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| OOAFDOEE_1 | 1661694 | 1662470 | CodY | 3.29e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OOAFDOEE_1 | 3393722 | 3394291 | ClpP | 2.44e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OOAFDOEE_1 | 0.0 | 1.0 | 0.0 |
| OOAFDOEE_2 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OOAFDOEE_1 | 1291473 | 1317785 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| OOAFDOEE_1 | 2505236 | 2535271 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600 |
Phigaro v2.4.0 (Database: Jan. 2024 release)