Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KBFLKOFI_1 | 2705592 | 2706425 | aadK | ARO:3002627 | aadK | 97.11 | 97.54 | AL009126.1 |
| KBFLKOFI_1 | 2688522 | 2689724 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| KBFLKOFI_1 | 2515272 | 2516441 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| KBFLKOFI_1 | 1405130 | 1405447 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| KBFLKOFI_1 | 1404792 | 1405130 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| KBFLKOFI_1 | 892536 | 895106 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| KBFLKOFI_1 | 581253 | 582899 | vmlR | ARO:3004476 | vmlR | 99.45 | 100.18 | NC_000964.3 |
| KBFLKOFI_1 | 324099 | 324692 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| KBFLKOFI_1 | 273543 | 274976 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.00 | JYFL01000006.1 |
| KBFLKOFI_1 | 260729 | 261649 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KBFLKOFI_1 | 260729 | 261649 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| KBFLKOFI_1 | 4166180 | 4167556 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.13 | 100.00 | X08034 |
| KBFLKOFI_1 | 2705592 | 2706446 | aadK | Streptomycin | 98.25 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KBFLKOFI_1 | 581256 | 582899 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.45 | 100.00 | WP_003234144.1 |
| KBFLKOFI_1 | 4166183 | 4167556 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_003242953.1 |
| KBFLKOFI_1 | 260729 | 261646 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| KBFLKOFI_1 | 2705595 | 2706446 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.18 | 100.00 | WP_003229862.1 |
| KBFLKOFI_1 | 4163694 | 4164212 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
| KBFLKOFI_1 | 2089874 | 2092471 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.76 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KBFLKOFI_1 | 2317971 | 2318612 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| KBFLKOFI_1 | 3673119 | 3674300 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| KBFLKOFI_1 | 3254329 | 3255114 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| KBFLKOFI_1 | 2035642 | 2037405 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| KBFLKOFI_1 | 3250493 | 3251431 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| KBFLKOFI_1 | 3671494 | 3672636 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| KBFLKOFI_1 | 3672655 | 3673104 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| KBFLKOFI_1 | 3243337 | 3250473 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391076 |
| KBFLKOFI_1 | 3253107 | 3254303 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.50 | 100.00 | NP_391079 |
| KBFLKOFI_1 | 3251459 | 3253078 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.16 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KBFLKOFI_1 | 132416 | 133552 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| KBFLKOFI_1 | 18600 | 19469 | pdxS | 3.11e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KBFLKOFI_1 | 1716587 | 1717363 | CodY | 3.40e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KBFLKOFI_1 | 3516547 | 3517116 | ClpP | 2.52e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KBFLKOFI_1 | 0.0 | 1.0 | 0.0 |
| KBFLKOFI_2 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KBFLKOFI_1 | 1263593 | 1285318 | False | Siphoviridae | VOG0796, VOG10227, VOG0720, VOG4564, VOG6778, VOG4555, VOG4553, VOG1125, VOG0815, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG4545, VOG4605, VOG4599, VOG5535 |
| KBFLKOFI_1 | 1350254 | 1375553 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG7895, VOG0753 |
| KBFLKOFI_1 | 2252171 | 2262098 | False | Myoviridae | VOG7567, VOG4570, VOG5722, VOG5779, VOG5721, VOG10940 |
| KBFLKOFI_1 | 2270699 | 2288009 | False | Siphoviridae | VOG0275, VOG9312, VOG9312, VOG6163, VOG4910, VOG3389, VOG5042, VOG9712, VOG4626, VOG3390, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)