Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IBMOCDID_1 | 2616967 | 2617821 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| IBMOCDID_1 | 2587443 | 2588645 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| IBMOCDID_1 | 2393373 | 2394542 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| IBMOCDID_1 | 1417566 | 1417883 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| IBMOCDID_1 | 1417228 | 1417566 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| IBMOCDID_1 | 955844 | 958414 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| IBMOCDID_1 | 627619 | 629262 | vmlR | ARO:3004476 | vmlR | 98.90 | 100.00 | NC_000964.3 |
| IBMOCDID_1 | 314655 | 316088 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| IBMOCDID_1 | 301840 | 302760 | mphK | ARO:3004541 | mphK | 96.41 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IBMOCDID_1 | 4105347 | 4106723 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.84 | 100.00 | D12567 |
| IBMOCDID_1 | 2616967 | 2617821 | aadK | Streptomycin | 98.60 | 100.00 | M26879 |
| IBMOCDID_1 | 301840 | 302760 | mph(K) | Spiramycin, Telithromycin | 96.64 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IBMOCDID_1 | 4105350 | 4106723 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_063856081.1 |
| IBMOCDID_1 | 627622 | 629262 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.90 | 100.00 | WP_003234144.1 |
| IBMOCDID_1 | 2616970 | 2617821 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| IBMOCDID_1 | 301840 | 302757 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.41 | 100.00 | WP_003246254.1 |
| IBMOCDID_1 | 4103160 | 4103678 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
| IBMOCDID_1 | 2093060 | 2095657 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.29 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IBMOCDID_1 | 3170166 | 3171104 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| IBMOCDID_1 | 3616852 | 3618033 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IBMOCDID_1 | 2190553 | 2191194 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| IBMOCDID_1 | 3172780 | 3173976 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.42 | 100.00 | NP_391079 |
| IBMOCDID_1 | 3174002 | 3174787 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| IBMOCDID_1 | 3615227 | 3616369 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| IBMOCDID_1 | 2047271 | 2049034 | dep/capD | 0.0 | Capsule | Immune modulation | 99.09 | 100.00 | NP_389723 |
| IBMOCDID_1 | 3616388 | 3616837 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| IBMOCDID_1 | 3171132 | 3172751 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.64 | 100.00 | NP_391078 |
| IBMOCDID_1 | 3163010 | 3170146 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.52 | 100.00 | NP_391076 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IBMOCDID_1 | 132339 | 133475 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| IBMOCDID_1 | 18602 | 19471 | pdxS | 3.07e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IBMOCDID_1 | 1728328 | 1729104 | CodY | 3.35e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IBMOCDID_1 | 3460144 | 3460713 | ClpP | 2.49e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IBMOCDID_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IBMOCDID_1 | 171285 | 185192 | False | Siphoviridae | VOG8484, VOG6242, VOG4618, VOG4567, VOG9315, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589 |
| IBMOCDID_1 | 676963 | 680518 | False | Unknown | VOG8483, VOG9667, VOG9667, VOG0650, VOG4552 |
| IBMOCDID_1 | 691124 | 714413 | False | Siphoviridae | VOG0198, VOG0796, VOG10227, VOG0720, VOG4564, VOG4555, VOG4553, VOG1125, VOG0815, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG4545, VOG4605, VOG4599, VOG5535, VOG4728, VOG0753, VOG0136, VOG4574 |
| IBMOCDID_1 | 1359796 | 1387377 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| IBMOCDID_1 | 3242744 | 3265824 | False | Siphoviridae | VOG4574, VOG0136, VOG5221, VOG7896, VOG3390, VOG4612, VOG4705, VOG3390, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4573, VOG4556, VOG2935, VOG1886, VOG4581, VOG4841 |
Phigaro v2.4.0 (Database: Jan. 2024 release)