Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DFFGOJJN_1 | 2524269 | 2525123 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| DFFGOJJN_1 | 2507170 | 2508372 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| DFFGOJJN_1 | 2328529 | 2329698 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| DFFGOJJN_1 | 1384966 | 1385283 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DFFGOJJN_1 | 1384628 | 1384966 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DFFGOJJN_1 | 886289 | 888859 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| DFFGOJJN_1 | 587575 | 589221 | vmlR | ARO:3004476 | vmlR | 98.91 | 100.18 | NC_000964.3 |
| DFFGOJJN_1 | 334816 | 335409 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| DFFGOJJN_1 | 284292 | 285725 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
| DFFGOJJN_1 | 271490 | 272410 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DFFGOJJN_1 | 271490 | 272410 | mph(K) | Spiramycin, Telithromycin | 99.24 | 100.00 | NC_000964 |
| DFFGOJJN_1 | 2524269 | 2525123 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DFFGOJJN_1 | 587578 | 589221 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.91 | 100.00 | WP_003234144.1 |
| DFFGOJJN_1 | 271490 | 272407 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| DFFGOJJN_1 | 2524272 | 2525123 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| DFFGOJJN_1 | 4083355 | 4083873 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| DFFGOJJN_1 | 2014142 | 2016730 | rphC | rifamycin-inactivating phosphotransferase RphC | INTERNAL_STOP | 80.25 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DFFGOJJN_1 | 3126837 | 3133973 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.71 | 100.00 | NP_391076 |
| DFFGOJJN_1 | 3593328 | 3593777 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| DFFGOJJN_1 | 3593792 | 3594973 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| DFFGOJJN_1 | 3137829 | 3138614 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| DFFGOJJN_1 | 3592167 | 3593309 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| DFFGOJJN_1 | 3136607 | 3137803 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| DFFGOJJN_1 | 1963558 | 1965321 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| DFFGOJJN_1 | 2127915 | 2128556 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| DFFGOJJN_1 | 3133993 | 3134931 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.30 | 100.00 | NP_391077 |
| DFFGOJJN_1 | 3134959 | 3136578 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DFFGOJJN_1 | 132496 | 133632 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DFFGOJJN_1 | 18600 | 19469 | pdxS | 3.05e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DFFGOJJN_1 | 1700481 | 1701257 | CodY | 3.33e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DFFGOJJN_1 | 3438821 | 3439390 | ClpP | 2.47e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DFFGOJJN_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DFFGOJJN_1 | 1327696 | 1355140 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| DFFGOJJN_1 | 1833575 | 1858980 | False | Siphoviridae | VOG0044, VOG0198, VOG0384, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4626, VOG4626, VOG4612, VOG6243, VOG4705, VOG0641, VOG4574 |
| DFFGOJJN_1 | 2972921 | 2999428 | False | Siphoviridae | VOG7531, VOG0705, VOG6116, VOG8440, VOG0602, VOG4574, VOG5221, VOG4705, VOG6243, VOG4612, VOG4626, VOG4619, VOG0801, VOG4545, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5601, VOG4553, VOG4573, VOG4556, VOG4544, VOG4581, VOG4841 |
| DFFGOJJN_1 | 3021380 | 3035365 | False | Myoviridae / Siphoviridae | VOG0026, VOG10653, VOG5324, VOG10937, VOG6546, VOG4134, VOG1563, VOG2215, VOG4551, VOG0025, VOG0044, VOG0189 |
| DFFGOJJN_1 | 3401926 | 3423188 | False | Siphoviridae | VOG0602, VOG4574, VOG5221, VOG4705, VOG6243, VOG4612, VOG4626, VOG4619, VOG0801, VOG11074, VOG4319, VOG9317, VOG0692, VOG2613, VOG3699, VOG5023, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)