Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 4 | 14.70 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FECJBJHA_1 | 2580947 | 2581801 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| FECJBJHA_1 | 2326235 | 2327404 | bmr | ARO:3003007 | bmr | 98.97 | 100.00 | M33768.1 |
| FECJBJHA_1 | 1456936 | 1457253 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| FECJBJHA_1 | 1456598 | 1456936 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| FECJBJHA_1 | 975494 | 978064 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| FECJBJHA_1 | 328629 | 330062 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FECJBJHA_1 | 4078590 | 4079966 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.35 | 100.00 | X08034 |
| FECJBJHA_1 | 2580949 | 2581801 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| FECJBJHA_1 | 302342 | 303261 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FECJBJHA_1 | 4078593 | 4079966 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_003242953.1 |
| FECJBJHA_1 | 2580950 | 2581801 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| FECJBJHA_1 | 646758 | 648398 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.62 | 100.00 | WP_003234144.1 |
| FECJBJHA_1 | 4076091 | 4076609 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 92.49 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FECJBJHA_1 | 3568288 | 3569469 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| FECJBJHA_1 | 3566663 | 3567805 | capA | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | NP_391469 |
| FECJBJHA_1 | 3567824 | 3568273 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| FECJBJHA_1 | 3147489 | 3148274 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| FECJBJHA_1 | 1984232 | 1985995 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| FECJBJHA_1 | 3136497 | 3143633 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.95 | 100.00 | NP_391076 |
| FECJBJHA_1 | 3146267 | 3147463 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391079 |
| FECJBJHA_1 | 3143653 | 3144591 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.62 | 100.00 | NP_391077 |
| FECJBJHA_1 | 3144619 | 3146238 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.90 | 100.00 | NP_391078 |
| FECJBJHA_1 | 2121028 | 2121669 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FECJBJHA_1 | 134366 | 135502 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| FECJBJHA_1 | 20245 | 21114 | pdxS | 5.37e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| FECJBJHA_1 | 1779644 | 1780420 | CodY | 3.35e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FECJBJHA_1 | 3413878 | 3414447 | ClpP | 2.48e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FECJBJHA_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FECJBJHA_1 | 698072 | 706432 | False | Siphoviridae | VOG4602, VOG4662, VOG9667, VOG9667, VOG0650, VOG4552, VOG4600, VOG1309, VOG4566, VOG0189 |
| FECJBJHA_1 | 708565 | 733159 | False | Siphoviridae | VOG0397, VOG0102, VOG0044, VOG3798, VOG0198, VOG7983, VOG0796, VOG10227, VOG0691, VOG0692, VOG9317, VOG4572, VOG2621, VOG0695, VOG0696, VOG0697, VOG6548, VOG10044, VOG0701, VOG9745, VOG4605, VOG4599, VOG5535, VOG10940, VOG0753, VOG0136, VOG4707, VOG1993 |
| FECJBJHA_1 | 1400043 | 1425187 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| FECJBJHA_1 | 2509659 | 2530757 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688 |
Phigaro v2.4.0 (Database: Jan. 2024 release)