Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KPHONNAB_1 | 2507443 | 2508297 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| KPHONNAB_1 | 2490344 | 2491546 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| KPHONNAB_1 | 2263578 | 2264747 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| KPHONNAB_1 | 1370297 | 1370614 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| KPHONNAB_1 | 1369959 | 1370297 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| KPHONNAB_1 | 890111 | 892681 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| KPHONNAB_1 | 586303 | 587949 | vmlR | ARO:3004476 | vmlR | 98.91 | 100.18 | NC_000964.3 |
| KPHONNAB_1 | 334718 | 335311 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| KPHONNAB_1 | 284215 | 285648 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| KPHONNAB_1 | 271402 | 272322 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KPHONNAB_1 | 4002670 | 4004046 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.84 | 100.00 | D12567 |
| KPHONNAB_1 | 2507443 | 2508297 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| KPHONNAB_1 | 271402 | 272322 | mph(K) | Spiramycin, Telithromycin | 98.26 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KPHONNAB_1 | 4002673 | 4004046 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_063856081.1 |
| KPHONNAB_1 | 586306 | 587949 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.91 | 100.00 | WP_003234144.1 |
| KPHONNAB_1 | 271402 | 272319 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| KPHONNAB_1 | 2507446 | 2508297 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| KPHONNAB_1 | 4000155 | 4000673 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| KPHONNAB_1 | 1951216 | 1953804 | rphC | rifamycin-inactivating phosphotransferase RphC | INTERNAL_STOP | 80.14 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KPHONNAB_1 | 3508067 | 3509248 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| KPHONNAB_1 | 3507603 | 3508052 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| KPHONNAB_1 | 3063429 | 3064214 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| KPHONNAB_1 | 3052437 | 3059573 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.31 | 100.00 | NP_391076 |
| KPHONNAB_1 | 3506442 | 3507584 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| KPHONNAB_1 | 3062207 | 3063403 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| KPHONNAB_1 | 1901752 | 1903515 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| KPHONNAB_1 | 2065011 | 2065652 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| KPHONNAB_1 | 3059593 | 3060531 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.30 | 100.00 | NP_391077 |
| KPHONNAB_1 | 3060559 | 3062178 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KPHONNAB_1 | 132415 | 133551 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| KPHONNAB_1 | 18600 | 19469 | pdxS | 3.01e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KPHONNAB_1 | 1680330 | 1681106 | CodY | 3.29e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KPHONNAB_1 | 3353356 | 3353925 | ClpP | 2.44e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KPHONNAB_1 | 0.0 | 1.0 | 0.0 |
| KPHONNAB_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KPHONNAB_1 | 1313745 | 1340472 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
| KPHONNAB_1 | 2435360 | 2465257 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600 |
| KPHONNAB_1 | 3228336 | 3255965 | False | Siphoviridae | VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG0204, VOG10223, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841, VOG0275, VOG0198, VOG0044, VOG0102, VOG0397, VOG5836, VOG0245, VOG1336 |
| KPHONNAB_1 | 3256321 | 3263176 | False | Siphoviridae | VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563 |
| KPHONNAB_2 | 27185 | 38629 | False | Unknown | VOG2352, VOG0397, VOG1198, VOG4561, VOG4714, VOG2352, VOG3851, VOG2725 |
| KPHONNAB_2 | 68528 | 83247 | False | Siphoviridae | VOG0221, VOG4581, VOG5819, VOG1886, VOG4556, VOG4568, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG5852 |
Phigaro v2.4.0 (Database: Jan. 2024 release)