Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DEBMKDGC_1 | 2603405 | 2604259 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| DEBMKDGC_1 | 2584717 | 2585919 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| DEBMKDGC_1 | 2358998 | 2360167 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DEBMKDGC_1 | 1367949 | 1368266 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| DEBMKDGC_1 | 1367611 | 1367949 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| DEBMKDGC_1 | 906364 | 908934 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| DEBMKDGC_1 | 598551 | 600176 | vmlR | ARO:3004476 | vmlR | 98.52 | 98.90 | NC_000964.3 |
| DEBMKDGC_1 | 340137 | 340730 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| DEBMKDGC_1 | 289602 | 291035 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| DEBMKDGC_1 | 276790 | 277710 | mphK | ARO:3004541 | mphK | 97.06 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DEBMKDGC_1 | 4117093 | 4118469 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.42 | 100.00 | D12567 |
| DEBMKDGC_1 | 2603405 | 2604259 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| DEBMKDGC_1 | 276790 | 277710 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DEBMKDGC_1 | 4117096 | 4118469 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_063856081.1 |
| DEBMKDGC_1 | 2603408 | 2604259 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| DEBMKDGC_1 | 598554 | 600176 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.52 | 98.90 | WP_003234144.1 |
| DEBMKDGC_1 | 276790 | 277707 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.06 | 100.00 | WP_003246254.1 |
| DEBMKDGC_1 | 4114563 | 4115081 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| DEBMKDGC_1 | 2051264 | 2053861 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.71 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DEBMKDGC_1 | 3156699 | 3158318 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.81 | 100.00 | NP_391078 |
| DEBMKDGC_1 | 3155733 | 3156671 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.79 | 100.00 | NP_391077 |
| DEBMKDGC_1 | 3603784 | 3604965 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| DEBMKDGC_1 | 2004568 | 2006331 | dep/capD | 0.0 | Capsule | Immune modulation | 99.60 | 100.00 | NP_389723 |
| DEBMKDGC_1 | 3159569 | 3160354 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| DEBMKDGC_1 | 3602159 | 3603301 | capA | 0.0 | Capsule | Immune modulation | 99.47 | 100.00 | NP_391469 |
| DEBMKDGC_1 | 2155987 | 2156628 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| DEBMKDGC_1 | 3603320 | 3603769 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| DEBMKDGC_1 | 3148577 | 3155713 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391076 |
| DEBMKDGC_1 | 3158347 | 3159543 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DEBMKDGC_1 | 132406 | 133542 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DEBMKDGC_1 | 18665 | 19534 | pdxS | 3.08e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DEBMKDGC_1 | 1678573 | 1679349 | CodY | 3.36e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DEBMKDGC_1 | 3452382 | 3452951 | ClpP | 2.49e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DEBMKDGC_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DEBMKDGC_1 | 1311796 | 1338117 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| DEBMKDGC_1 | 2534352 | 2553063 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG10989, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685 |
| DEBMKDGC_1 | 3229328 | 3253557 | False | Siphoviridae | VOG4574, VOG0641, VOG4705, VOG6243, VOG4612, VOG4358, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG4841, VOG0531 |
Phigaro v2.4.0 (Database: Jan. 2024 release)