Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KAKCBLMI_1 | 2623462 | 2624316 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| KAKCBLMI_1 | 2604773 | 2605975 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| KAKCBLMI_1 | 2380338 | 2381507 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| KAKCBLMI_1 | 1394068 | 1394385 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| KAKCBLMI_1 | 1393730 | 1394068 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| KAKCBLMI_1 | 933040 | 935610 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| KAKCBLMI_1 | 592513 | 594156 | vmlR | ARO:3004476 | vmlR | 98.72 | 100.00 | NC_000964.3 |
| KAKCBLMI_1 | 341137 | 341730 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| KAKCBLMI_1 | 290576 | 292009 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| KAKCBLMI_1 | 277758 | 278678 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KAKCBLMI_1 | 4103318 | 4104694 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.49 | 100.00 | D12567 |
| KAKCBLMI_1 | 2623464 | 2624316 | aadK | Streptomycin | 99.30 | 99.77 | M26879 |
| KAKCBLMI_1 | 277758 | 278678 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KAKCBLMI_1 | 4103321 | 4104694 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_063856081.1 |
| KAKCBLMI_1 | 277758 | 278675 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| KAKCBLMI_1 | 592516 | 594156 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.72 | 100.00 | WP_003234144.1 |
| KAKCBLMI_1 | 2623465 | 2624316 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| KAKCBLMI_1 | 4100797 | 4101315 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| KAKCBLMI_1 | 2072941 | 2075538 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.17 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KAKCBLMI_1 | 3181094 | 3181879 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| KAKCBLMI_1 | 3177258 | 3178196 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| KAKCBLMI_1 | 3595292 | 3596473 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| KAKCBLMI_1 | 3170102 | 3177238 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.58 | 100.00 | NP_391076 |
| KAKCBLMI_1 | 3593667 | 3594809 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| KAKCBLMI_1 | 2180942 | 2181583 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| KAKCBLMI_1 | 3594828 | 3595277 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| KAKCBLMI_1 | 3179872 | 3181068 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| KAKCBLMI_1 | 2022940 | 2024703 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| KAKCBLMI_1 | 3178224 | 3179843 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.33 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KAKCBLMI_1 | 132402 | 133538 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| KAKCBLMI_1 | 18665 | 19534 | pdxS | 3.07e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KAKCBLMI_1 | 1704178 | 1704954 | CodY | 3.35e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KAKCBLMI_1 | 3433119 | 3433688 | ClpP | 2.48e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KAKCBLMI_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KAKCBLMI_1 | 657525 | 683145 | False | Siphoviridae | VOG0796, VOG10227, VOG0720, VOG4564, VOG4555, VOG4553, VOG1125, VOG0815, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG2621, VOG0725, VOG4545, VOG4605, VOG4599, VOG5535, VOG4728, VOG0753, VOG0136, VOG4574, VOG1993, VOG2703, VOG10522 |
| KAKCBLMI_1 | 1337799 | 1363929 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| KAKCBLMI_1 | 2553556 | 2576158 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773 |
Phigaro v2.4.0 (Database: Jan. 2024 release)