Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AILPGBCH_1 | 2482390 | 2483244 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| AILPGBCH_1 | 2465291 | 2466493 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| AILPGBCH_1 | 2286639 | 2287808 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| AILPGBCH_1 | 1385613 | 1385930 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| AILPGBCH_1 | 1385275 | 1385613 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| AILPGBCH_1 | 886200 | 888770 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| AILPGBCH_1 | 587482 | 589128 | vmlR | ARO:3004476 | vmlR | 98.91 | 100.18 | NC_000964.3 |
| AILPGBCH_1 | 334749 | 335342 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| AILPGBCH_1 | 284254 | 285687 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| AILPGBCH_1 | 271442 | 272362 | mphK | ARO:3004541 | mphK | 99.35 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| AILPGBCH_1 | 271442 | 272362 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| AILPGBCH_1 | 2482390 | 2483244 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AILPGBCH_1 | 271442 | 272359 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.35 | 100.00 | WP_003246254.1 |
| AILPGBCH_1 | 587485 | 589128 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.91 | 100.00 | WP_003234144.1 |
| AILPGBCH_1 | 2482393 | 2483244 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| AILPGBCH_1 | 4000190 | 4000708 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| AILPGBCH_1 | 1972264 | 1974852 | rphC | rifamycin-inactivating phosphotransferase RphC | INTERNAL_STOP | 80.25 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AILPGBCH_1 | 3081736 | 3088872 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.71 | 100.00 | NP_391076 |
| AILPGBCH_1 | 3509942 | 3510391 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| AILPGBCH_1 | 3510406 | 3511587 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| AILPGBCH_1 | 3092728 | 3093513 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| AILPGBCH_1 | 3508781 | 3509923 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| AILPGBCH_1 | 3091506 | 3092702 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| AILPGBCH_1 | 1921682 | 1923445 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| AILPGBCH_1 | 2086035 | 2086676 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| AILPGBCH_1 | 3088892 | 3089830 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.30 | 100.00 | NP_391077 |
| AILPGBCH_1 | 3089858 | 3091477 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AILPGBCH_1 | 132497 | 133633 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| AILPGBCH_1 | 18600 | 19469 | pdxS | 2.99e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| AILPGBCH_1 | 1701132 | 1701908 | CodY | 3.27e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| AILPGBCH_1 | 3355450 | 3356019 | ClpP | 2.42e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AILPGBCH_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AILPGBCH_1 | 1328345 | 1355787 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| AILPGBCH_1 | 2646935 | 2672275 | False | Siphoviridae | VOG5216, VOG10051, VOG6116, VOG7508, VOG0602, VOG4666, VOG5221, VOG4705, VOG6243, VOG4612, VOG4626, VOG4619, VOG0801, VOG4545, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5601, VOG4553, VOG4573, VOG4556, VOG4544, VOG4581, VOG4841, VOG6430 |
| AILPGBCH_1 | 2692891 | 2709393 | False | Myoviridae / Siphoviridae | VOG0026, VOG8296, VOG10937, VOG5753, VOG7255, VOG0052, VOG1563, VOG11003, VOG2215, VOG4551, VOG0025, VOG0044, VOG0189, VOG5325, VOG6168, VOG0152, VOG0586 |
Phigaro v2.4.0 (Database: Jan. 2024 release)