Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 7 | 16.43 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GADBFPBG_10 | 1631865 | 1632719 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| GADBFPBG_10 | 1591440 | 1592642 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| GADBFPBG_10 | 1410802 | 1411971 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| GADBFPBG_10 | 423903 | 424220 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| GADBFPBG_10 | 423565 | 423903 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| GADBFPBG_16 | 297372 | 299942 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| GADBFPBG_18 | 108611 | 109531 | mphK | ARO:3004541 | mphK | 97.06 | 100.00 | CP010314.1 |
| GADBFPBG_18 | 95281 | 96720 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| GADBFPBG_18 | 45559 | 46152 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| GADBFPBG_17 | 29102 | 30748 | vmlR | ARO:3004476 | vmlR | 98.91 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GADBFPBG_10 | 1631865 | 1632719 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| GADBFPBG_18 | 108611 | 109531 | mph(K) | Spiramycin, Telithromycin | 97.07 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GADBFPBG_17 | 29102 | 30745 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.91 | 100.00 | WP_003234144.1 |
| GADBFPBG_10 | 1631868 | 1632719 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| GADBFPBG_18 | 108614 | 109531 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.06 | 100.00 | WP_003246254.1 |
| GADBFPBG_10 | 1117711 | 1120308 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.52 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GADBFPBG_15 | 113001 | 113786 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| GADBFPBG_15 | 523388 | 524569 | capB | 0.0 | Capsule | Immune modulation | 99.41 | 100.00 | NP_391471 |
| GADBFPBG_15 | 522924 | 523373 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| GADBFPBG_10 | 1211209 | 1211850 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| GADBFPBG_15 | 521763 | 522905 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| GADBFPBG_15 | 111779 | 112975 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| GADBFPBG_15 | 109165 | 110103 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.72 | 100.00 | NP_391077 |
| GADBFPBG_15 | 102009 | 109145 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.61 | 100.00 | NP_391076 |
| GADBFPBG_10 | 1071979 | 1073742 | dep/capD | 0.0 | Capsule | Immune modulation | 98.53 | 100.00 | NP_389723 |
| GADBFPBG_15 | 110131 | 111750 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.46 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GADBFPBG_19 | 28103 | 26967 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| GADBFPBG_21 | 4356 | 5225 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| GADBFPBG_10 | 735749 | 736525 | CodY | 1.68e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GADBFPBG_15 | 366945 | 367514 | ClpP | 6.38e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| GADBFPBG_10 | 275728 | 276120 | spxA1 | 1.32e-69 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| GADBFPBG_10 | 275728 | 276120 | spx | 7.87e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| GADBFPBG_20 | 34709 | 34467 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| GADBFPBG_17 | 87283 | 87086 | CspA | 1.05e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GADBFPBG_10 | 0.0 | 1.0 | 0.0 |
| GADBFPBG_15 | 0.0 | 1.0 | 0.0 |
| GADBFPBG_16 | 0.0 | 1.0 | 0.0 |
| GADBFPBG_17 | 0.0 | 1.0 | 0.0 |
| GADBFPBG_18 | 0.0 | 1.0 | 0.0 |
| GADBFPBG_19 | 0.0 | 1.0 | 0.0 |
| GADBFPBG_20 | 0.0 | 1.0 | 0.0 |
| GADBFPBG_21 | 0.0 | 1.0 | 0.0 |
| GADBFPBG_22 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GADBFPBG_10 | 367256 | 393697 | False | Myoviridae | VOG4602, VOG9667, VOG10599, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| GADBFPBG_16 | 12001 | 19590 | False | Siphoviridae | VOG0275, VOG4602, VOG9607, VOG9667, VOG4603, VOG0650, VOG4552, VOG4603 |
| GADBFPBG_16 | 27565 | 52913 | False | Siphoviridae | VOG0198, VOG0796, VOG10227, VOG0720, VOG4564, VOG4555, VOG4553, VOG1125, VOG0815, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG1323, VOG9745, VOG4605, VOG4599, VOG5535, VOG4728, VOG0641, VOG4574 |
Phigaro v2.4.0 (Database: Jan. 2024 release)