Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KBFHEIFM_1 | 2635769 | 2636623 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| KBFHEIFM_1 | 2617044 | 2618246 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| KBFHEIFM_1 | 2423867 | 2425036 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| KBFHEIFM_1 | 1393347 | 1393664 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| KBFHEIFM_1 | 1393009 | 1393347 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| KBFHEIFM_1 | 933978 | 936548 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| KBFHEIFM_1 | 636984 | 638627 | vmlR | ARO:3004476 | vmlR | 98.17 | 100.00 | NC_000964.3 |
| KBFHEIFM_1 | 391771 | 392364 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| KBFHEIFM_1 | 341246 | 342679 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| KBFHEIFM_1 | 328433 | 329353 | mphK | ARO:3004541 | mphK | 97.39 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KBFHEIFM_1 | 4184088 | 4185464 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.78 | 100.00 | D12567 |
| KBFHEIFM_1 | 2635769 | 2636623 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| KBFHEIFM_1 | 328433 | 329353 | mph(K) | Spiramycin, Telithromycin | 97.61 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KBFHEIFM_1 | 4184091 | 4185464 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_063856081.1 |
| KBFHEIFM_1 | 2635772 | 2636623 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| KBFHEIFM_1 | 636987 | 638627 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.17 | 100.00 | WP_003234144.1 |
| KBFHEIFM_1 | 328433 | 329350 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.39 | 100.00 | WP_003246254.1 |
| KBFHEIFM_1 | 4181595 | 4182113 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| KBFHEIFM_1 | 2118052 | 2120649 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KBFHEIFM_1 | 3272860 | 3273798 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| KBFHEIFM_1 | 2221074 | 2221715 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| KBFHEIFM_1 | 3273826 | 3275445 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.81 | 100.00 | NP_391078 |
| KBFHEIFM_1 | 3265704 | 3272840 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391076 |
| KBFHEIFM_1 | 3691845 | 3693026 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| KBFHEIFM_1 | 3691381 | 3691830 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| KBFHEIFM_1 | 2066214 | 2067977 | dep/capD | 0.0 | Capsule | Immune modulation | 99.55 | 100.00 | NP_389723 |
| KBFHEIFM_1 | 3275474 | 3276670 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.42 | 100.00 | NP_391079 |
| KBFHEIFM_1 | 3276696 | 3277481 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| KBFHEIFM_1 | 3690220 | 3691362 | capA | 0.0 | Capsule | Immune modulation | 99.12 | 100.00 | NP_391469 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KBFHEIFM_1 | 132455 | 133591 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| KBFHEIFM_1 | 18660 | 19529 | pdxS | 3.13e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KBFHEIFM_1 | 1735984 | 1736760 | CodY | 3.42e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KBFHEIFM_1 | 3529975 | 3530544 | ClpP | 2.54e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KBFHEIFM_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KBFHEIFM_1 | 168780 | 174783 | False | Siphoviridae | VOG4603, VOG8241, VOG3549, VOG1878, VOG0083, VOG1309, VOG1292 |
| KBFHEIFM_1 | 185381 | 212343 | False | Myoviridae | VOG1328, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG10095, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG0906, VOG7718, VOG7895 |
| KBFHEIFM_1 | 1336348 | 1362051 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| KBFHEIFM_1 | 2768949 | 2795640 | False | Siphoviridae | VOG4574, VOG0641, VOG7896, VOG3390, VOG4612, VOG4705, VOG3390, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886, VOG4581, VOG4841, VOG2545, VOG0376, VOG0198 |
| KBFHEIFM_1 | 3141622 | 3162774 | False | Siphoviridae | VOG0136, VOG0753, VOG10940, VOG5535, VOG4599, VOG4605, VOG0725, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG0815, VOG1125, VOG4553, VOG4555, VOG4564, VOG0720, VOG10227 |
Phigaro v2.4.0 (Database: Jan. 2024 release)