Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BLIJHABK_3 | 312702 | 313622 | mphK | ARO:3004541 | mphK | 99.35 | 100.00 | CP010314.1 |
| BLIJHABK_3 | 299378 | 300811 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
| BLIJHABK_3 | 249675 | 250268 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| BLIJHABK_1 | 184384 | 185553 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BLIJHABK_8 | 34096 | 34953 | aadK | ARO:3002627 | aadK | 97.54 | 100.35 | AL009126.1 |
| BLIJHABK_5 | 27441 | 30011 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| BLIJHABK_8 | 16952 | 18154 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| BLIJHABK_2 | 4188 | 4526 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BLIJHABK_2 | 3871 | 4188 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BLIJHABK_25 | 140 | 1786 | vmlR | ARO:3004476 | vmlR | 97.99 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BLIJHABK_3 | 312702 | 313622 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| BLIJHABK_8 | 34102 | 34953 | aadK | Streptomycin | 98.36 | 99.65 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BLIJHABK_3 | 312705 | 313622 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.35 | 100.00 | WP_003246254.1 |
| BLIJHABK_25 | 143 | 1786 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 97.99 | 100.00 | WP_003234144.1 |
| BLIJHABK_8 | 34102 | 34953 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| BLIJHABK_7 | 39785 | 40303 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 94.80 | 100.00 | WP_003242546.1 |
| BLIJHABK_13 | 25645 | 28242 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.76 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BLIJHABK_1 | 382234 | 382875 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| BLIJHABK_17 | 22067 | 23248 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| BLIJHABK_10 | 112995 | 113780 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.98 | 100.00 | NP_391080 |
| BLIJHABK_17 | 23263 | 23712 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| BLIJHABK_13 | 75890 | 77653 | dep/capD | 0.0 | Capsule | Immune modulation | 98.64 | 100.00 | NP_389723 |
| BLIJHABK_10 | 109158 | 110096 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.51 | 100.00 | NP_391077 |
| BLIJHABK_10 | 102002 | 109138 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.47 | 100.00 | NP_391076 |
| BLIJHABK_17 | 23731 | 24873 | capA | 0.0 | Capsule | Immune modulation | 98.42 | 100.00 | NP_391469 |
| BLIJHABK_10 | 110124 | 111743 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.03 | 100.00 | NP_391078 |
| BLIJHABK_10 | 111772 | 112968 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.16 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BLIJHABK_15 | 28014 | 26878 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BLIJHABK_19 | 11277 | 10408 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BLIJHABK_4 | 93758 | 94534 | CodY | 2.55e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BLIJHABK_6 | 125164 | 124595 | ClpP | 1.71e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BLIJHABK_2 | 174056 | 173664 | spxA1 | 2.97e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BLIJHABK_2 | 174056 | 173664 | spx | 1.77e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BLIJHABK_16 | 20573 | 20815 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BLIJHABK_3 | 42436 | 42239 | CspA | 3.55e-32 | cold shock protein | 92.42 | 100.00 | CAA62903 |
| BLIJHABK_1 | 371004 | 370810 | CspD | 1.21e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| BLIJHABK_1 | 371004 | 370807 | CspB | 2.50e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| BLIJHABK_3 | 42436 | 42242 | CspR | 1.23e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BLIJHABK_1 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_2 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_3 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_4 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_5 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_6 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_7 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_8 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_9 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_10 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_11 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_12 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_13 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_14 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_15 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_16 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_17 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_18 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_19 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_20 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_21 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_22 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_23 | 1.0 | 0.0 | 1.0 |
| BLIJHABK_25 | 0.0 | 1.0 | 0.0 |
| BLIJHABK_26 | 1.0 | 0.0 | 1.0 |
| BLIJHABK_27 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BLIJHABK_2 | 34371 | 61875 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG1510, VOG2752, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)