Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 2 | 4 | 4.90 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KJPFCEJH_1 | 1301574 | 1302623 | clbA | ARO:3002814 | clbA | 99.43 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KJPFCEJH_1 | 1301588 | 1302623 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.80 | 98.67 | KR610408 |
| KJPFCEJH_1 | 3366595 | 3367971 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 87.00 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KJPFCEJH_1 | 1301577 | 1302623 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 99.43 | 100.00 | WP_012116915.1 |
| KJPFCEJH_1 | 3366595 | 3367965 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.21 | 99.78 | WP_003242953.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KJPFCEJH_1 | 2350990 | 2352171 | capB | 0.0 | Capsule | Immune modulation | 81.96 | 99.66 | NP_391471 |
| KJPFCEJH_1 | 2352186 | 2352635 | capC | 7.31e-112 | Capsule | Immune modulation | 83.37 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KJPFCEJH_1 | 1734483 | 1733347 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| KJPFCEJH_1 | 1849157 | 1848288 | pdxS | 3.54e-150 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| KJPFCEJH_1 | 192944 | 192168 | CodY | 5.02e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KJPFCEJH_1 | 2764870 | 2765742 | lipA (SAUSA300_0829) | 1.36e-161 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KJPFCEJH_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KJPFCEJH_1 | 570216 | 596586 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
| KJPFCEJH_1 | 1187742 | 1213251 | False | Siphoviridae | VOG3490, VOG6422, VOG10230, VOG0136, VOG0753, VOG5535, VOG4599, VOG4605, VOG4545, VOG0725, VOG2621, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG0815, VOG1125, VOG4553, VOG4555, VOG4564, VOG0720, VOG10439, VOG0796, VOG4681, VOG3244, VOG4972, VOG3631 |
| KJPFCEJH_1 | 1224410 | 1229861 | False | Siphoviridae | VOG4552, VOG0650, VOG11075, VOG9667, VOG1561, VOG4602 |
| KJPFCEJH_1 | 2903444 | 2912852 | False | Siphoviridae | VOG3390, VOG4705, VOG8610, VOG4995, VOG3699, VOG0221, VOG5023, VOG0720, VOG4555, VOG4553, VOG4713 |
| KJPFCEJH_1 | 3307100 | 3332103 | False | Siphoviridae | VOG6242, VOG0198, VOG0275, VOG0531, VOG4841, VOG0713, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG1266, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641 |
| KJPFCEJH_1 | 3786417 | 3812345 | False | Siphoviridae | VOG4574, VOG4660, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG1799, VOG0275, VOG7930, VOG8610, VOG5008, VOG3390, VOG7718, VOG5534, VOG6940, VOG9713 |
Phigaro v2.4.0 (Database: Jan. 2024 release)