Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 6 | 0 | 1 | 6.08 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GGNPBOHD_22 | 247813 | 248778 | CfxA2 | ARO:3003002 | CfxA2 | 99.69 | 100.00 | AF118110.1 |
| GGNPBOHD_33 | 94246 | 94983 | ErmB | ARO:3000375 | ErmB | 97.96 | 98.79 | AF242872.1 |
| GGNPBOHD_12 | 69030 | 70661 | ErmF | ARO:3000498 | ErmF | 99.62 | 204.14 | M17124.1 |
| GGNPBOHD_12 | 67681 | 68817 | tet(X) | ARO:3000205 | tet(X) | 99.74 | 97.42 | M37699.1 |
| GGNPBOHD_11 | 55680 | 57605 | tet(Q) | ARO:3000191 | tet(Q) | 96.41 | 97.56 | Z21523.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GGNPBOHD_12 | 69030 | 69830 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | M17808 |
| GGNPBOHD_12 | 67651 | 68817 | tet(X) | Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.91 | 100.00 | M37699 |
| GGNPBOHD_11 | 55680 | 57605 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.84 | 100.00 | L33696 |
| GGNPBOHD_22 | 247813 | 248778 | cfxA3 | Ampicillin | 99.79 | 100.00 | AF472622 |
| GGNPBOHD_22 | 247813 | 248778 | cfxA4 | Unknown Beta-lactam | 99.79 | 100.00 | AY769933 |
| GGNPBOHD_22 | 247813 | 248778 | cfxA5 | Unknown Beta-lactam | 99.79 | 100.00 | AY769934 |
| GGNPBOHD_22 | 247813 | 248778 | cfxA | Cefoxitin, Ampicillin | 99.79 | 100.00 | U38243 |
| GGNPBOHD_33 | 94246 | 94983 | erm(B) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.73 | 100.00 | U18931 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GGNPBOHD_12 | 69033 | 69830 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | EXACTX | 100.00 | 100.00 | WP_002682030.1 |
| GGNPBOHD_12 | 67651 | 68814 | tet(X2) | tetracycline-inactivating monooxygenase Tet(X2) | BLASTX | 99.74 | 100.00 | WP_008651082.1 |
| GGNPBOHD_22 | 247813 | 248775 | cfxA | CfxA family broad-spectrum class A beta-lactamase | BLASTX | 99.69 | 100.00 | WP_004339683.1 |
| GGNPBOHD_11 | 55680 | 57602 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.53 | 100.00 | WP_063856407.1 |
| GGNPBOHD_33 | 94249 | 94983 | erm(B) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | BLASTX | 99.18 | 100.00 | WP_002292226.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GGNPBOHD_19 | 12423 | 10843 | GroEL | 0.0 | folding of newly synthesized proteins, preventing misfolding and aggregation | 86.91 | 96.70 | BAA04222 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| GGNPBOHD_46 | 837 | 1516 | repUS2 | 682 / 681 | 98.53 | BFU30316 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GGNPBOHD_1 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_2 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_3 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_4 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_5 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_6 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_7 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_8 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_9 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_10 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_11 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_12 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_13 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_14 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_15 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_16 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_17 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_18 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_19 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_20 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_21 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_22 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_23 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_24 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_25 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_26 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_27 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_28 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_29 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_30 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_31 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_32 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_33 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_34 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_35 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_36 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_37 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_38 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_39 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_40 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_41 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_43 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_44 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_45 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_46 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_47 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_48 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_49 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_50 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_51 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_52 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_53 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_54 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_55 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_56 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_57 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_58 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_59 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_60 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_61 | 1.0 | 0.0 | 1.0 |
| GGNPBOHD_62 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_63 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_64 | 0.0 | 1.0 | 0.0 |
| GGNPBOHD_65 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GGNPBOHD_2 | 108111 | 116567 | False | Siphoviridae | VOG7238, |
| GGNPBOHD_12 | 56621 | 56980 | False | Unknown | |
| GGNPBOHD_25 | 12168 | 16927 | True | Siphoviridae | VOG10059, |
| GGNPBOHD_33 | 115159 | 119781 | False | Siphoviridae | VOG7108, |
Phigaro v2.4.0 (Database: Jan. 2024 release)