Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 7 | 0 | 0 | 7.00 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MPCKHBHH_33 | 501417 | 502382 | CfxA2 | ARO:3003002 | CfxA2 | 100.00 | 100.00 | AF118110.1 |
| MPCKHBHH_22 | 487401 | 488138 | ErmB | ARO:3000375 | ErmB | 97.96 | 98.79 | AF242872.1 |
| MPCKHBHH_43 | 166387 | 167277 | CblA-1 | ARO:3002999 | CblA-1 | 99.66 | 100.00 | GQ343019.1 |
| MPCKHBHH_44 | 32810 | 34441 | ErmF | ARO:3000498 | ErmF | 99.62 | 204.14 | M17124.1 |
| MPCKHBHH_44 | 31461 | 32597 | tet(X) | ARO:3000205 | tet(X) | 99.74 | 97.42 | M37699.1 |
| MPCKHBHH_44 | 6023 | 7948 | tet(Q) | ARO:3000191 | tet(Q) | 96.41 | 97.56 | Z21523.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MPCKHBHH_44 | 32810 | 33610 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | M17808 |
| MPCKHBHH_44 | 31431 | 32597 | tet(X) | Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.91 | 100.00 | M37699 |
| MPCKHBHH_33 | 501417 | 502382 | cfxA3 | Ampicillin | 99.90 | 100.00 | AF472622 |
| MPCKHBHH_33 | 501417 | 502382 | cfxA4 | Unknown Beta-lactam | 99.90 | 100.00 | AY769933 |
| MPCKHBHH_33 | 501417 | 502382 | cfxA5 | Unknown Beta-lactam | 99.90 | 100.00 | AY769934 |
| MPCKHBHH_33 | 501417 | 502382 | cfxA | Cefoxitin, Ampicillin | 99.90 | 100.00 | U38243 |
| MPCKHBHH_44 | 6023 | 7948 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.84 | 100.00 | L33696 |
| MPCKHBHH_22 | 487401 | 488138 | erm(B) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.73 | 100.00 | U18931 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MPCKHBHH_33 | 501420 | 502382 | cfxA | CfxA family broad-spectrum class A beta-lactamase | EXACTX | 100.00 | 100.00 | WP_004339683.1 |
| MPCKHBHH_44 | 32813 | 33610 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | EXACTX | 100.00 | 100.00 | WP_002682030.1 |
| MPCKHBHH_44 | 31431 | 32594 | tet(X2) | tetracycline-inactivating monooxygenase Tet(X2) | BLASTX | 99.74 | 100.00 | WP_008651082.1 |
| MPCKHBHH_43 | 166390 | 167277 | cblA | CblA family class A beta-lactamase | BLASTX | 99.66 | 100.00 | WP_005827792.1 |
| MPCKHBHH_44 | 6026 | 7948 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.53 | 100.00 | WP_063856407.1 |
| MPCKHBHH_22 | 487401 | 488135 | erm(B) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | BLASTX | 99.18 | 100.00 | WP_002292226.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| MPCKHBHH_31 | 186 | 865 | repUS2 | 682 / 681 | 98.53 | BFU30316 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MPCKHBHH_1 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_2 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_3 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_4 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_5 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_6 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_7 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_8 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_9 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_10 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_11 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_12 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_13 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_14 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_15 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_16 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_17 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_18 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_19 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_20 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_21 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_22 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_24 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_25 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_26 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_27 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_28 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_29 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_30 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_31 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_32 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_33 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_34 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_35 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_36 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_37 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_38 | 1.0 | 0.0 | 1.0 |
| MPCKHBHH_39 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_40 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_41 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_42 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_43 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_44 | 0.0 | 1.0 | 0.0 |
| MPCKHBHH_45 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MPCKHBHH_44 | 262673 | 268436 | True | Unknown | VOG8607, |
Phigaro v2.4.0 (Database: Jan. 2024 release)