Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 0 | 0 | 10.00 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCEKDNAD_71 | 150964 | 151602 | ErmG | ARO:3000522 | ErmG | 99.52 | 86.89 | L42817.1 |
| MCEKDNAD_11 | 129845 | 131819 | tet(Q) | ARO:3000191 | tet(Q) | 100.00 | 97.56 | Z21523.1 |
| MCEKDNAD_1 | 121103 | 122308 | Mef(En2) | ARO:3004659 | Mef(En2) | 99.50 | 100.00 | AF251288.1 |
| MCEKDNAD_1 | 46324 | 47529 | Mef(En2) | ARO:3004659 | Mef(En2) | 99.50 | 100.00 | AF251288.1 |
| MCEKDNAD_23 | 342 | 1307 | CfxA3 | ARO:3003003 | CfxA3 | 100.00 | 100.00 | AF472622.2 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MCEKDNAD_23 | 342 | 1307 | cfxA3 | Ampicillin | 100.00 | 100.00 | AF472622 |
| MCEKDNAD_11 | 129845 | 131770 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 100.00 | 100.00 | Z21523 |
| MCEKDNAD_19 | 69068 | 69934 | ant(6)-Ia | Streptomycin | 99.88 | 100.00 | KF864551 |
| MCEKDNAD_71 | 150867 | 151602 | erm(G) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.18 | 100.00 | M15332 |
| MCEKDNAD_71 | 152374 | 153837 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 97.54 | 100.00 | AF274302 |
| MCEKDNAD_71 | 153963 | 155177 | mef(A) | Erythromycin, Azithromycin | 94.24 | 99.75 | AF227520 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCEKDNAD_19 | 69071 | 69934 | aadE | aminoglycoside 6-adenylyltransferase AadE | EXACTX | 100.00 | 100.00 | WP_001255868.1 |
| MCEKDNAD_23 | 342 | 1304 | cfxA3 | extended-spectrum class A beta-lactamase CfxA3 | EXACTX | 100.00 | 100.00 | WP_004348227.1 |
| MCEKDNAD_1 | 120569 | 121078 | lnu(AN2) | lincosamide nucleotidyltransferase Lnu(AN2) | EXACTX | 100.00 | 100.00 | WP_004308783.1 |
| MCEKDNAD_1 | 45790 | 46299 | lnu(AN2) | lincosamide nucleotidyltransferase Lnu(AN2) | EXACTX | 100.00 | 100.00 | WP_004308783.1 |
| MCEKDNAD_11 | 129848 | 131770 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | EXACTX | 100.00 | 100.00 | WP_002560998.1 |
| MCEKDNAD_71 | 150976 | 151602 | erm(G) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(G) | PARTIALX | 99.52 | 85.66 | WP_014387027.1 |
| MCEKDNAD_1 | 121106 | 122308 | mef(En2) | macrolide efflux MFS transporter Mef(En2) | BLASTX | 99.50 | 100.00 | WP_063853729.1 |
| MCEKDNAD_1 | 46327 | 47529 | mef(En2) | macrolide efflux MFS transporter Mef(En2) | BLASTX | 99.50 | 100.00 | WP_063853729.1 |
| MCEKDNAD_71 | 152377 | 153837 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | BLASTX | 97.13 | 100.00 | WP_000420313.1 |
| MCEKDNAD_71 | 153954 | 155177 | mef(A) | macrolide efflux MFS transporter Mef(A) | BLASTX | 96.57 | 100.00 | WP_012102963.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MCEKDNAD_1 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_2 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_3 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_4 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_5 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_6 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_7 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_8 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_9 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_10 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_11 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_12 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_13 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_14 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_15 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_16 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_17 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_18 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_19 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_20 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_21 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_22 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_23 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_24 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_25 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_26 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_27 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_28 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_29 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_30 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_31 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_32 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_33 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_34 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_35 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_36 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_37 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_38 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_39 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_40 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_41 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_42 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_43 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_44 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_45 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_46 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_47 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_48 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_49 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_50 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_52 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_53 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_54 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_55 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_56 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_57 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_58 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_59 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_60 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_61 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_62 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_63 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_64 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_65 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_66 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_67 | 1.0 | 0.0 | 1.0 |
| MCEKDNAD_68 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_69 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_70 | 0.0 | 1.0 | 0.0 |
| MCEKDNAD_71 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MCEKDNAD_44 | 73003 | 81634 | False | Unknown | VOG0226, |
| MCEKDNAD_44 | 94140 | 103328 | False | Unknown | VOG1031, |
Phigaro v2.4.0 (Database: Jan. 2024 release)