safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
BMAELMJO_1	2730910	2731764	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
BMAELMJO_1	2712201	2713403	blt	ARO:3003006	blt	100.00	100.00	L32599.1
BMAELMJO_1	2489882	2491051	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
BMAELMJO_1	1376458	1376775	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
BMAELMJO_1	1376120	1376458	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
BMAELMJO_1	916376	918946	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
BMAELMJO_1	604333	605976	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
BMAELMJO_1	338755	339348	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
BMAELMJO_1	288252	289685	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
BMAELMJO_1	275437	276357	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
BMAELMJO_1	2730910	2731764	aadK	Streptomycin	100.00	100.00	M26879
BMAELMJO_1	275437	276357	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
BMAELMJO_1	4182928	4184304	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
BMAELMJO_1	2730913	2731764	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
BMAELMJO_1	275437	276354	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
BMAELMJO_1	4180410	4180928	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
BMAELMJO_1	4182931	4184304	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
BMAELMJO_1	604336	605976	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
BMAELMJO_1	2046568	2049162	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
BMAELMJO_1	3693068	3694210	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
BMAELMJO_1	3694693	3695874	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
BMAELMJO_1	3694229	3694678	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
BMAELMJO_1	1999910	2001673	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
BMAELMJO_1	3286732	3287517	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
BMAELMJO_1	3282896	3283834	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
BMAELMJO_1	3285510	3286706	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
BMAELMJO_1	3283862	3285481	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
BMAELMJO_1	3275740	3282876	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
BMAELMJO_1	2290536	2291177	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BMAELMJO_1	132473	133609	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
BMAELMJO_1	18665	19534	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
BMAELMJO_1	1689722	1690498	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
BMAELMJO_1	3541456	3542025	ClpP	2.53e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BMAELMJO_1	0.0	1.0	0.0
BMAELMJO_2	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
BMAELMJO_1	1320173	1346281	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
BMAELMJO_1	2662272	2692516	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600
BMAELMJO_2	22981	36079	False	Unknown	VOG4549, VOG2725, VOG3851, VOG2352, VOG4714, VOG4561, VOG1198, VOG0397, VOG2352, VOG11080
BMAELMJO_2	55493	77301	False	Siphoviridae	VOG3616, VOG1024, VOG10944, VOG9244, VOG4599, VOG4605, VOG6163, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581

Phigaro v2.4.0 (Database: Jan. 2024 release)