Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EHMLMMCA_1 | 3844649 | 3845569 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
| EHMLMMCA_1 | 3831318 | 3832751 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| EHMLMMCA_1 | 3781596 | 3782189 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| EHMLMMCA_1 | 3529181 | 3530824 | vmlR | ARO:3004476 | vmlR | 98.90 | 100.00 | NC_000964.3 |
| EHMLMMCA_1 | 3231273 | 3233843 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| EHMLMMCA_1 | 2733632 | 2733970 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| EHMLMMCA_1 | 2733315 | 2733632 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| EHMLMMCA_1 | 1746194 | 1747363 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| EHMLMMCA_1 | 1524765 | 1525967 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| EHMLMMCA_1 | 1506384 | 1507238 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EHMLMMCA_1 | 26508 | 27884 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.49 | 100.00 | D12567 |
| EHMLMMCA_1 | 1506384 | 1507236 | aadK | Streptomycin | 99.30 | 99.77 | M26879 |
| EHMLMMCA_1 | 3844649 | 3845569 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EHMLMMCA_1 | 26508 | 27881 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_063856081.1 |
| EHMLMMCA_1 | 3844652 | 3845569 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| EHMLMMCA_1 | 3529181 | 3530821 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.90 | 100.00 | WP_003234144.1 |
| EHMLMMCA_1 | 1506384 | 1507235 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| EHMLMMCA_1 | 29887 | 30405 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| EHMLMMCA_1 | 2052169 | 2054766 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.17 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EHMLMMCA_1 | 948743 | 949528 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| EHMLMMCA_1 | 952426 | 953364 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| EHMLMMCA_1 | 534140 | 535321 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| EHMLMMCA_1 | 953384 | 960520 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.58 | 100.00 | NP_391076 |
| EHMLMMCA_1 | 535804 | 536946 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| EHMLMMCA_1 | 1946123 | 1946764 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| EHMLMMCA_1 | 535336 | 535785 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| EHMLMMCA_1 | 949554 | 950750 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| EHMLMMCA_1 | 2102996 | 2104759 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| EHMLMMCA_1 | 950779 | 952398 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.33 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EHMLMMCA_1 | 3990924 | 3989788 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| EHMLMMCA_1 | 4104659 | 4103790 | pdxS | 3.06e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EHMLMMCA_1 | 2423520 | 2422744 | CodY | 3.34e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EHMLMMCA_1 | 697494 | 696925 | ClpP | 2.48e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EHMLMMCA_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EHMLMMCA_1 | 1545621 | 1575657 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4691, VOG4862 |
| EHMLMMCA_1 | 2763771 | 2789901 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| EHMLMMCA_1 | 2852336 | 2872639 | False | Siphoviridae | VOG4574, VOG0136, VOG0753, VOG10940, VOG5535, VOG4599, VOG4605, VOG9745, VOG0701, VOG10044, VOG6548, VOG0697, VOG0696, VOG0695, VOG2621, VOG4572, VOG9317, VOG0692, VOG0691, VOG10227, VOG0796 |
Phigaro v2.4.0 (Database: Jan. 2024 release)