Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 7 | 17.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MBIEGOPH_1 | 1683717 | 1684571 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| MBIEGOPH_1 | 1664539 | 1665741 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| MBIEGOPH_1 | 1436707 | 1437876 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MBIEGOPH_1 | 466751 | 467068 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MBIEGOPH_1 | 466413 | 466751 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MBIEGOPH_14 | 259692 | 262262 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| MBIEGOPH_15 | 196048 | 197691 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| MBIEGOPH_16 | 115471 | 116910 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.42 | JYFL01000006.1 |
| MBIEGOPH_16 | 102655 | 103575 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MBIEGOPH_18 | 34481 | 35857 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | X08034 |
| MBIEGOPH_1 | 1683719 | 1684571 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| MBIEGOPH_16 | 102655 | 103575 | mph(K) | Spiramycin, Telithromycin | 97.50 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MBIEGOPH_18 | 34481 | 35854 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| MBIEGOPH_15 | 196051 | 197691 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| MBIEGOPH_16 | 102655 | 103572 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| MBIEGOPH_1 | 1683720 | 1684571 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| MBIEGOPH_18 | 37856 | 38374 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 89.60 | 100.00 | WP_003242546.1 |
| MBIEGOPH_1 | 1132987 | 1135584 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MBIEGOPH_1 | 1237544 | 1238185 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| MBIEGOPH_12 | 529004 | 530185 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| MBIEGOPH_12 | 527379 | 528521 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| MBIEGOPH_12 | 114489 | 115274 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| MBIEGOPH_12 | 110653 | 111591 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391077 |
| MBIEGOPH_12 | 528540 | 528989 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| MBIEGOPH_12 | 113267 | 114463 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| MBIEGOPH_1 | 1086403 | 1088166 | dep/capD | 0.0 | Capsule | Immune modulation | 99.09 | 100.00 | NP_389723 |
| MBIEGOPH_12 | 103497 | 110633 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.09 | 100.00 | NP_391076 |
| MBIEGOPH_12 | 111619 | 113238 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.52 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MBIEGOPH_19 | 28002 | 26866 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MBIEGOPH_2 | 4345 | 5214 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MBIEGOPH_1 | 776651 | 777427 | CodY | 1.73e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MBIEGOPH_12 | 367646 | 368215 | ClpP | 4.16e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MBIEGOPH_1 | 275641 | 276033 | spxA1 | 1.36e-69 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MBIEGOPH_1 | 275641 | 276033 | spx | 8.10e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| MBIEGOPH_20 | 20581 | 20823 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MBIEGOPH_15 | 145297 | 145494 | CspA | 9.79e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MBIEGOPH_1 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_2 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_3 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_4 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_5 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_6 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_8 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_12 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_14 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_15 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_16 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_17 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_18 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_19 | 0.0 | 1.0 | 0.0 |
| MBIEGOPH_20 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MBIEGOPH_1 | 325037 | 345296 | False | Siphoviridae | VOG5574, VOG0198, VOG7983, VOG0796, VOG10227, VOG0691, VOG0692, VOG9317, VOG4572, VOG2621, VOG0695, VOG0696, VOG0697, VOG0698, VOG10044, VOG0701, VOG9745, VOG4605, VOG4599, VOG5535, VOG10940 |
| MBIEGOPH_1 | 409102 | 436925 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| MBIEGOPH_1 | 1614697 | 1637255 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773 |
Phigaro v2.4.0 (Database: Jan. 2024 release)