Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 26 | 32 | 41.23 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ECDENMBE_10 | 409 | 1377 | cbpD | 0.0 | Autolysin | Exoenzyme | 100.00 | 100.00 | WP_082302295 |
| ECDENMBE_14 | 31826 | 32419 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| ECDENMBE_2 | 76817 | 78076 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 99.84 | 100.00 | WP_011681445 |
| ECDENMBE_14 | 31826 | 32419 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| ECDENMBE_14 | 31826 | 32419 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| ECDENMBE_2 | 84080 | 84886 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | WP_011681437 |
| ECDENMBE_2 | 83139 | 84080 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 99.68 | 100.00 | WP_011227430 |
| ECDENMBE_2 | 83139 | 84080 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011681438 |
| ECDENMBE_39 | 225 | 1883 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| ECDENMBE_2 | 84080 | 84886 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011227429 |
| ECDENMBE_2 | 78073 | 79050 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | WP_011681444 |
| ECDENMBE_2 | 81994 | 83142 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 99.48 | 100.00 | WP_011227431 |
| ECDENMBE_2 | 81994 | 83142 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | WP_011681439 |
| ECDENMBE_2 | 79068 | 80036 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | WP_011681443 |
| ECDENMBE_2 | 87473 | 89218 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 99.31 | 100.00 | WP_011681434 |
| ECDENMBE_19 | 25541 | 26302 | srtA | 0.0 | Sortase A | Exoenzyme | 99.21 | 100.00 | - |
| ECDENMBE_14 | 30957 | 31826 | rfbA | 0.0 | Capsule | Immune modulation | 99.19 | 100.00 | WP_011681286 |
| ECDENMBE_3 | 52010 | 52800 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.74 | 100.00 | - |
| ECDENMBE_20 | 13310 | 14041 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011681176 |
| ECDENMBE_20 | 13310 | 14041 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011226048 |
| ECDENMBE_20 | 13310 | 14041 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011227248 |
| ECDENMBE_2 | 81099 | 81875 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.94 | 100.00 | WP_011681440 |
| ECDENMBE_2 | 87473 | 89218 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 97.88 | 100.00 | WP_011227426 |
| ECDENMBE_2 | 80038 | 80753 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 97.77 | 99.86 | WP_011681442 |
| ECDENMBE_20 | 11849 | 13309 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.67 | 100.00 | WP_011681177 |
| ECDENMBE_20 | 15550 | 16611 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 97.17 | 77.63 | WP_011226045 |
| ECDENMBE_20 | 11849 | 13309 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.78 | 100.21 | WP_011226049 |
| ECDENMBE_2 | 75847 | 76698 | rfbD | 0.0 | Capsule | Immune modulation | 96.48 | 100.00 | WP_014621820 |
| ECDENMBE_20 | 14752 | 15487 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.47 | 98.13 | WP_011227246 |
| ECDENMBE_20 | 14752 | 15489 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 96.21 | 99.60 | WP_011681174 |
| ECDENMBE_20 | 11849 | 13309 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.17 | 100.21 | WP_041827043 |
| ECDENMBE_20 | 14752 | 15489 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 95.66 | 99.60 | WP_011226046 |
| ECDENMBE_20 | 15550 | 16611 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.92 | 77.63 | WP_011681173 |
| ECDENMBE_20 | 14050 | 14742 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 91.64 | 99.86 | WP_011226047 |
| ECDENMBE_20 | 14050 | 14742 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 91.50 | 99.86 | WP_011227247 |
| ECDENMBE_1 | 92701 | 94004 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| ECDENMBE_36 | 188 | 1198 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| ECDENMBE_20 | 14050 | 14742 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 86.78 | 99.57 | WP_011681175 |
| ECDENMBE_2 | 84886 | 86109 | SGO_RS05025 | 0.0 | Capsule | Immune modulation | 84.35 | 99.92 | WP_012000441 |
| ECDENMBE_2 | 84080 | 84886 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 83.15 | 100.00 | WP_012000440 |
| ECDENMBE_14 | 32848 | 33894 | rfbB | 0.0 | Capsule | Immune modulation | 80.80 | 100.00 | WP_009659423 |
| ECDENMBE_55 | 25 | 1308 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
| ECDENMBE_2 | 84886 | 85623 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 97.97 | 61.35 | WP_011227428 |
| ECDENMBE_2 | 84886 | 85623 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 97.70 | 61.35 | WP_011681436 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ECDENMBE_19 | 22854 | 21871 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| ECDENMBE_28 | 6615 | 7688 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| ECDENMBE_1 | 177686 | 176718 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| ECDENMBE_6 | 52426 | 54729 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| ECDENMBE_33 | 11235 | 8989 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| ECDENMBE_22 | 14612 | 16093 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.50 | 100.00 | ABJ54480 |
| ECDENMBE_23 | 20940 | 21938 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| ECDENMBE_69 | 1413 | 511 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.03 | 92.90 | ABF36968 |
| ECDENMBE_2 | 27441 | 28592 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| ECDENMBE_2 | 62809 | 64797 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| ECDENMBE_14 | 9802 | 11532 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| ECDENMBE_5 | 63426 | 61948 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| ECDENMBE_5 | 48231 | 46339 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| ECDENMBE_1 | 94004 | 92703 | Eno | 0.0 | enolase | 85.98 | 99.77 | ABP90468 |
| ECDENMBE_33 | 6064 | 7773 | PGM | 0.0 | phosphoglucomutase | 83.71 | 99.65 | ACH87041 |
| ECDENMBE_4 | 19034 | 20044 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| ECDENMBE_13 | 9068 | 7707 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.02 | 99.56 | ABP90420 |
| ECDENMBE_4 | 69299 | 67407 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| ECDENMBE_1 | 108438 | 107548 | bglB | 1.36e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| ECDENMBE_11 | 45779 | 46717 | SP_0676 | 2.05e-169 | transcription facot | 81.47 | 97.20 | AAK74821 |
| ECDENMBE_33 | 8797 | 7940 | metF | 7.54e-167 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| ECDENMBE_3 | 24363 | 23659 | covR | 4.47e-132 | responder protein | 85.11 | 100.00 | AAK71319 |
| ECDENMBE_18 | 29342 | 28671 | rr01 | 8.57e-128 | response regulator | 84.82 | 99.56 | CAB54566 |
| ECDENMBE_3 | 24363 | 23659 | walR | 6.02e-122 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| ECDENMBE_33 | 2111 | 1461 | CiaR | 2.47e-119 | two-component response regulator | 86.64 | 96.88 | AAK74935 |
| ECDENMBE_8 | 9676 | 8990 | CovR | 3.16e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| ECDENMBE_33 | 2111 | 1461 | ciaR | 1.05e-116 | two component system response regulator | 84.33 | 96.02 | AIK75708 |
| ECDENMBE_23 | 22214 | 22609 | SpxA2 | 4.51e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| ECDENMBE_16 | 13053 | 13340 | spr0479 | 2.93e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| ECDENMBE_10 | 42265 | 42519 | spr1327 | 7.76e-46 | essential gene | 95.29 | 100.00 | AAL00131 |
| ECDENMBE_5 | 14621 | 14884 | spr0175 | 3.35e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| ECDENMBE_5 | 15424 | 15714 | spr0177 | 1.97e-37 | essential gene | 81.00 | 98.02 | AAK98981 |
| ECDENMBE_7 | 144 | 644 | RopE (M5005_Spy_1611) | 1.66e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| ECDENMBE_6 | 52426 | 54723 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| ECDENMBE_4 | 54896 | 53877 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| ECDENMBE_64 | 233 | 1180 | rep37 | 948 / 948 | 93.04 | AF177168 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ECDENMBE_1 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_2 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_3 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_4 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_5 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_6 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_7 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_8 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_9 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_10 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_11 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_12 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_13 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_14 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_15 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_16 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_17 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_18 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_19 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_20 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_21 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_22 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_23 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_24 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_25 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_26 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_27 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_28 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_29 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_30 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_31 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_32 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_33 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_34 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_35 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_36 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_37 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_38 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_39 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_40 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_41 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_42 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_43 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_44 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_45 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_46 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_47 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_48 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_49 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_50 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_52 | 1.0 | 0.0 | 1.0 |
| ECDENMBE_53 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_54 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_55 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_56 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_57 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_58 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_59 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_60 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_61 | 1.0 | 0.0 | 1.0 |
| ECDENMBE_62 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_63 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_64 | 1.0 | 0.0 | 1.0 |
| ECDENMBE_65 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_66 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_67 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_68 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_69 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_70 | 1.0 | 0.0 | 1.0 |
| ECDENMBE_71 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_72 | 0.0 | 1.0 | 0.0 |
| ECDENMBE_73 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)