Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 8 | 18.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BOCKNHKG_1 | 526661 | 526999 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BOCKNHKG_1 | 526344 | 526661 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BOCKNHKG_13 | 440508 | 441362 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| BOCKNHKG_13 | 421499 | 422701 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| BOCKNHKG_13 | 220446 | 221615 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BOCKNHKG_20 | 162258 | 162851 | tmrB | ARO:3003059 | tmrB | 98.98 | 100.00 | AL009126.3 |
| BOCKNHKG_20 | 111700 | 113139 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| BOCKNHKG_20 | 98881 | 99801 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
| BOCKNHKG_24 | 57011 | 58654 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| BOCKNHKG_19 | 27458 | 30028 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BOCKNHKG_13 | 440510 | 441362 | aadK | Streptomycin | 99.30 | 99.77 | M26879 |
| BOCKNHKG_23 | 2942 | 4318 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.26 | 100.00 | D12567 |
| BOCKNHKG_20 | 98881 | 99801 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BOCKNHKG_13 | 440511 | 441362 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| BOCKNHKG_20 | 98881 | 99798 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| BOCKNHKG_24 | 57014 | 58654 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| BOCKNHKG_23 | 2942 | 4315 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.25 | 100.00 | WP_063856081.1 |
| BOCKNHKG_23 | 6263 | 6781 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| BOCKNHKG_25 | 54261 | 56858 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BOCKNHKG_2 | 113182 | 113967 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| BOCKNHKG_2 | 110312 | 111931 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.51 | 100.00 | NP_391078 |
| BOCKNHKG_2 | 523861 | 525042 | capB | 0.0 | Capsule | Immune modulation | 99.41 | 100.00 | NP_391471 |
| BOCKNHKG_2 | 523397 | 523846 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| BOCKNHKG_2 | 111960 | 113156 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| BOCKNHKG_13 | 21038 | 21679 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| BOCKNHKG_2 | 522236 | 523378 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| BOCKNHKG_2 | 109346 | 110284 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| BOCKNHKG_25 | 8503 | 10266 | dep/capD | 0.0 | Capsule | Immune modulation | 98.75 | 100.00 | NP_389723 |
| BOCKNHKG_2 | 102190 | 109326 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.61 | 100.00 | NP_391076 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BOCKNHKG_4 | 31798 | 32934 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| BOCKNHKG_9 | 11312 | 10443 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BOCKNHKG_1 | 213249 | 212473 | CodY | 7.73e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BOCKNHKG_2 | 364689 | 365258 | ClpP | 5.41e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BOCKNHKG_1 | 677544 | 677152 | spxA1 | 6.08e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BOCKNHKG_1 | 677544 | 677152 | spx | 3.63e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BOCKNHKG_5 | 34801 | 34559 | SpoVG | 7.53e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BOCKNHKG_24 | 9006 | 9203 | CspA | 3.78e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BOCKNHKG_13 | 33640 | 33834 | CspD | 5.40e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BOCKNHKG_1 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_2 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_3 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_4 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_5 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_6 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_7 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_8 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_9 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_10 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_11 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_12 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_13 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_14 | 1.0 | 0.0 | 1.0 |
| BOCKNHKG_15 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_16 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_17 | 1.0 | 0.0 | 1.0 |
| BOCKNHKG_18 | 1.0 | 0.0 | 1.0 |
| BOCKNHKG_19 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_20 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_21 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_22 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_23 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_24 | 0.0 | 1.0 | 0.0 |
| BOCKNHKG_25 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BOCKNHKG_1 | 556865 | 583661 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| BOCKNHKG_21 | 17061 | 27896 | False | Siphoviridae | VOG1198, VOG0918, VOG4551, VOG0025, VOG7476, VOG0322 |
| BOCKNHKG_21 | 66824 | 86711 | False | Siphoviridae | VOG5047, VOG7483, VOG0094, VOG0937, VOG7567, VOG4570, VOG4581, VOG5722, VOG5779, VOG5721, VOG10940, VOG7134, VOG9867, VOG6036, VOG5495, VOG9219, VOG9713, VOG6940 |
Phigaro v2.4.0 (Database: Jan. 2024 release)