Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 5 | 15.68 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AHJOJEOH_3 | 3228052 | 3229221 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| AHJOJEOH_3 | 3046453 | 3047655 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| AHJOJEOH_3 | 3028038 | 3028892 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| AHJOJEOH_3 | 940476 | 940793 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| AHJOJEOH_3 | 940138 | 940476 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| AHJOJEOH_3 | 482018 | 484588 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| AHJOJEOH_2 | 294661 | 295254 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| AHJOJEOH_2 | 246789 | 248222 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| AHJOJEOH_1 | 79015 | 80391 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.77 | 100.00 | D12567 |
| AHJOJEOH_3 | 3028038 | 3028890 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| AHJOJEOH_2 | 233985 | 234904 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AHJOJEOH_1 | 79018 | 80391 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| AHJOJEOH_3 | 3028038 | 3028889 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| AHJOJEOH_1 | 76522 | 77040 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AHJOJEOH_3 | 2437597 | 2438535 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| AHJOJEOH_3 | 1981289 | 1982470 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| AHJOJEOH_3 | 1982953 | 1984095 | capA | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391469 |
| AHJOJEOH_3 | 1982485 | 1982934 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| AHJOJEOH_3 | 2433925 | 2434710 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| AHJOJEOH_3 | 1489876 | 1491639 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| AHJOJEOH_3 | 2438555 | 2445691 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.78 | 100.00 | NP_391076 |
| AHJOJEOH_3 | 2434736 | 2435932 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| AHJOJEOH_3 | 3444779 | 3445420 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
| AHJOJEOH_3 | 2435950 | 2437569 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AHJOJEOH_2 | 107484 | 108620 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| AHJOJEOH_2 | 8977 | 9846 | pdxS | 4.43e-150 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| AHJOJEOH_3 | 1254592 | 1255368 | CodY | 2.94e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| AHJOJEOH_3 | 2136320 | 2135751 | ClpP | 2.18e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| AHJOJEOH_2 | 41014 | 41256 | SpoVG | 4.64e-38 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| AHJOJEOH_5 | 312 | 505 | Col(SD853) | 194 / 194 | 98.97 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AHJOJEOH_1 | 0.0 | 1.0 | 0.0 |
| AHJOJEOH_2 | 0.0 | 1.0 | 0.0 |
| AHJOJEOH_3 | 0.0 | 1.0 | 0.0 |
| AHJOJEOH_4 | 1.0 | 0.0 | 1.0 |
| AHJOJEOH_5 | 1.0 | 0.0 | 1.0 |
| AHJOJEOH_6 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AHJOJEOH_3 | 885062 | 910926 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705, VOG0753 |
| AHJOJEOH_3 | 2146564 | 2174658 | False | Siphoviridae | VOG10599, VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG5601, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG4545, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0753, VOG4574, VOG0602, VOG7508 |
| AHJOJEOH_3 | 2864411 | 2898117 | False | Siphoviridae | VOG7438, VOG1047, VOG0021, VOG1565, VOG0026, VOG4609, VOG0327, VOG4548, VOG0198, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG5221, VOG0136 |
| AHJOJEOH_3 | 3245586 | 3261223 | False | Unknown | VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG8610, VOG5008 |
Phigaro v2.4.0 (Database: Jan. 2024 release)