Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 6 | 0 | 1 | 6.08 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BNBMOHIO_11 | 671948 | 673873 | tet(Q) | ARO:3000191 | tet(Q) | 89.08 | 97.56 | Z21523.1 |
| BNBMOHIO_11 | 412050 | 413996 | tet(Q) | ARO:3000191 | tet(Q) | 96.24 | 98.63 | Z21523.1 |
| BNBMOHIO_16 | 24809 | 26014 | Mef(En2) | ARO:3004659 | Mef(En2) | 99.50 | 100.00 | AF251288.1 |
| BNBMOHIO_29 | 8233 | 9198 | CfxA2 | ARO:3003002 | CfxA2 | 99.69 | 100.00 | AF118110.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BNBMOHIO_29 | 8233 | 9198 | cfxA3 | Ampicillin | 99.90 | 100.00 | AF472622 |
| BNBMOHIO_29 | 8233 | 9198 | cfxA4 | Unknown Beta-lactam | 99.90 | 100.00 | AY769933 |
| BNBMOHIO_11 | 412084 | 413996 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 97.12493465760585 | 99.33 | L33696 |
| BNBMOHIO_9 | 56881 | 57669 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 83.44 | 98.25 | M17808 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BNBMOHIO_29 | 8236 | 9198 | cfxA | CfxA family broad-spectrum class A beta-lactamase | BLASTX | 99.69 | 100.00 | WP_004339683.1 |
| BNBMOHIO_16 | 24809 | 26011 | mef(En2) | macrolide efflux MFS transporter Mef(En2) | BLASTX | 99.50 | 100.00 | WP_063853729.1 |
| BNBMOHIO_16 | 26039 | 26548 | lnu(AN2) | lincosamide nucleotidyltransferase Lnu(AN2) | BLASTX | 99.41 | 100.00 | WP_004308783.1 |
| BNBMOHIO_11 | 412083 | 413996 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.37 | 99.53 | WP_063856409.1 |
| BNBMOHIO_11 | 671951 | 673873 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 89.55 | 100.00 | WP_063856407.1 |
| BNBMOHIO_9 | 56882 | 57673 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | BLASTX | 80.30 | 99.25 | WP_002682030.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BNBMOHIO_10 | 85427 | 87007 | GroEL | 0.0 | folding of newly synthesized proteins, preventing misfolding and aggregation | 86.91 | 96.70 | BAA04222 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| BNBMOHIO_38 | 341 | 1020 | repUS2 | 682 / 681 | 98.53 | BFU30316 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BNBMOHIO_1 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_2 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_3 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_4 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_5 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_6 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_7 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_8 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_9 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_10 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_11 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_12 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_13 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_14 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_15 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_16 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_17 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_18 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_19 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_20 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_21 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_22 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_23 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_24 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_25 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_26 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_27 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_28 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_29 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_30 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_31 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_32 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_33 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_34 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_35 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_36 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_37 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_38 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_39 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_40 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_41 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_42 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_43 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_44 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_45 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_46 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_47 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_48 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_50 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_52 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_53 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_54 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_55 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_57 | 1.0 | 0.0 | 1.0 |
| BNBMOHIO_59 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_64 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_68 | 0.0 | 1.0 | 0.0 |
| BNBMOHIO_69 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BNBMOHIO_2 | 7125 | 19474 | False | Unknown | VOG1198, |
| BNBMOHIO_22 | 332161 | 339907 | False | Siphoviridae | VOG7238, |
| BNBMOHIO_69 | 71799 | 86753 | False | Myoviridae | VOG2149, |
Phigaro v2.4.0 (Database: Jan. 2024 release)