Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 0 | 1 | 3.16 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FBBOLAIF_50 | 11239 | 13164 | tet(Q) | ARO:3000191 | tet(Q) | 96.41 | 97.56 | Z21523.1 |
| FBBOLAIF_28 | 1809 | 2609 | ErmF | ARO:3000498 | ErmF | 98.87 | 100.00 | M17124.1 |
| FBBOLAIF_26 | 63 | 1028 | CfxA2 | ARO:3003002 | CfxA2 | 100.00 | 100.00 | AF118110.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FBBOLAIF_26 | 63 | 1028 | cfxA3 | Ampicillin | 99.90 | 100.00 | AF472622 |
| FBBOLAIF_26 | 63 | 1028 | cfxA4 | Unknown Beta-lactam | 99.90 | 100.00 | AY769933 |
| FBBOLAIF_26 | 63 | 1028 | cfxA5 | Unknown Beta-lactam | 99.90 | 100.00 | AY769934 |
| FBBOLAIF_26 | 63 | 1028 | cfxA | Cefoxitin, Ampicillin | 99.90 | 100.00 | U38243 |
| FBBOLAIF_50 | 11239 | 13164 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.84 | 100.00 | L33696 |
| FBBOLAIF_28 | 1809 | 2609 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.50 | 100.00 | M17808 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FBBOLAIF_26 | 63 | 1025 | cfxA | CfxA family broad-spectrum class A beta-lactamase | EXACTX | 100.00 | 100.00 | WP_004339683.1 |
| FBBOLAIF_50 | 11242 | 13164 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.53 | 100.00 | WP_063856407.1 |
| FBBOLAIF_28 | 1809 | 2606 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | BLASTX | 99.25 | 100.00 | WP_002682030.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FBBOLAIF_10 | 43232 | 44812 | GroEL | 0.0 | folding of newly synthesized proteins, preventing misfolding and aggregation | 86.91 | 96.70 | BAA04222 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| FBBOLAIF_34 | 2010 | 2689 | repUS2 | 682 / 681 | 99.27 | BFU30316 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FBBOLAIF_1 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_2 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_3 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_4 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_5 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_6 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_7 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_8 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_9 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_10 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_11 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_12 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_13 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_14 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_15 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_16 | 1.0 | 0.0 | 1.0 |
| FBBOLAIF_17 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_18 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_19 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_20 | 1.0 | 0.0 | 1.0 |
| FBBOLAIF_21 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_22 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_23 | 1.0 | 0.0 | 1.0 |
| FBBOLAIF_24 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_25 | 1.0 | 0.0 | 1.0 |
| FBBOLAIF_26 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_27 | 1.0 | 0.0 | 1.0 |
| FBBOLAIF_28 | 1.0 | 0.0 | 1.0 |
| FBBOLAIF_29 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_31 | 1.0 | 0.0 | 1.0 |
| FBBOLAIF_32 | 1.0 | 0.0 | 1.0 |
| FBBOLAIF_33 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_34 | 1.0 | 0.0 | 1.0 |
| FBBOLAIF_35 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_36 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_37 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_38 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_44 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_47 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_48 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_49 | 0.0 | 1.0 | 0.0 |
| FBBOLAIF_50 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FBBOLAIF_2 | 63373 | 71548 | False | Siphoviridae | VOG7108, |
| FBBOLAIF_44 | 38946 | 43166 | False | Myoviridae | VOG1353, |
Phigaro v2.4.0 (Database: Jan. 2024 release)