Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 2 | 6 | 7.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FGIHAILE_9 | 334704 | 335750 | clbA | ARO:3002814 | clbA | 92.77 | 99.71 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FGIHAILE_9 | 334704 | 335747 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.90 | 99.33 | KR610408 |
| FGIHAILE_8 | 361985 | 363361 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 87.36 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FGIHAILE_9 | 334704 | 335747 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 92.24 | 99.71 | WP_012116915.1 |
| FGIHAILE_8 | 361985 | 363355 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 87.09 | 99.78 | WP_003242953.1 |
| FGIHAILE_9 | 332435 | 332953 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 82.66 | 100.00 | WP_003242546.1 |
| FGIHAILE_6 | 1112651 | 1115248 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FGIHAILE_7 | 376623 | 377804 | capB | 0.0 | Capsule | Immune modulation | 81.87 | 99.66 | NP_391471 |
| FGIHAILE_7 | 377819 | 378268 | capC | 3.62e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FGIHAILE_12 | 31774 | 32910 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| FGIHAILE_14 | 4574 | 5443 | pdxS | 6.52e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| FGIHAILE_6 | 740621 | 741397 | CodY | 1.05e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FGIHAILE_6 | 260544 | 260936 | spxA1 | 1.66e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| FGIHAILE_9 | 318202 | 318399 | CspA | 5.86e-32 | cold shock protein | 92.42 | 100.00 | CAA62903 |
| FGIHAILE_8 | 702843 | 702649 | CspD | 2.07e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| FGIHAILE_8 | 702843 | 702646 | CspB | 4.30e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| FGIHAILE_9 | 318202 | 318396 | CspR | 2.91e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FGIHAILE_1 | 0.0 | 1.0 | 0.0 |
| FGIHAILE_6 | 0.0 | 1.0 | 0.0 |
| FGIHAILE_7 | 0.0 | 1.0 | 0.0 |
| FGIHAILE_8 | 0.0 | 1.0 | 0.0 |
| FGIHAILE_9 | 0.0 | 1.0 | 0.0 |
| FGIHAILE_10 | 0.0 | 1.0 | 0.0 |
| FGIHAILE_11 | 0.0 | 1.0 | 0.0 |
| FGIHAILE_12 | 0.0 | 1.0 | 0.0 |
| FGIHAILE_13 | 0.0 | 1.0 | 0.0 |
| FGIHAILE_14 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FGIHAILE_6 | 311539 | 318043 | False | Siphoviridae | VOG4995, VOG3699, VOG0221, VOG7734, VOG0720, VOG4555, VOG4572 |
| FGIHAILE_6 | 390366 | 415753 | False | Myoviridae | VOG9667, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| FGIHAILE_6 | 808960 | 832697 | False | Siphoviridae | VOG8537, VOG10230, VOG4660, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG2090, VOG2091, VOG0275, VOG7930, VOG8610, VOG4705, VOG3390, VOG7718 |
| FGIHAILE_6 | 1058538 | 1096180 | False | Siphoviridae | VOG0275, VOG4602, VOG9667, VOG6495, VOG4552, VOG0650, VOG7438, VOG1047, VOG0021, VOG1565, VOG0026, VOG4609, VOG0327, VOG4548, VOG0198, VOG0531, VOG4581, VOG4544, VOG5051, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4772, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641 |
| FGIHAILE_7 | 760179 | 785232 | False | Siphoviridae | VOG6495, VOG1655, VOG10069, VOG8909, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG4705, VOG0753, VOG4574 |
| FGIHAILE_7 | 932009 | 960526 | False | Siphoviridae | VOG5327, VOG1183, VOG0275, VOG5023, VOG3699, VOG2613, VOG0692, VOG4555, VOG3676, VOG5361, VOG2565, VOG11074, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG5221, VOG4824, VOG0602, VOG6116, VOG10051 |
| FGIHAILE_10 | 13163 | 27120 | False | Siphoviridae | VOG4602, VOG8483, VOG9667, VOG11075, VOG0650, VOG4552, VOG4600, VOG4752, VOG1309, VOG9093, VOG0189, VOG0044, VOG0536, VOG1336, VOG4658, VOG8569, VOG0397, VOG6581 |
| FGIHAILE_10 | 29492 | 58559 | False | Siphoviridae | VOG5447, VOG4972, VOG4681, VOG0796, VOG10227, VOG0720, VOG4564, VOG6778, VOG4555, VOG1329, VOG2621, VOG5872, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG4545, VOG4605, VOG4599, VOG3390, VOG5535, VOG0753, VOG0136, VOG4574 |
Phigaro v2.4.0 (Database: Jan. 2024 release)