Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 7 | 17.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DOEOEBLJ_11 | 695400 | 696569 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DOEOEBLJ_11 | 465109 | 466311 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| DOEOEBLJ_11 | 446289 | 447143 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| DOEOEBLJ_21 | 428092 | 428409 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DOEOEBLJ_21 | 427754 | 428092 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DOEOEBLJ_24 | 266734 | 269304 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| DOEOEBLJ_25 | 196104 | 197747 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| DOEOEBLJ_26 | 111146 | 112585 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| DOEOEBLJ_26 | 98330 | 99250 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DOEOEBLJ_26 | 98330 | 99250 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| DOEOEBLJ_28 | 34746 | 36122 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | X08034 |
| DOEOEBLJ_11 | 446289 | 447141 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DOEOEBLJ_26 | 98330 | 99247 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| DOEOEBLJ_28 | 34746 | 36119 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| DOEOEBLJ_25 | 196107 | 197747 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| DOEOEBLJ_11 | 446289 | 447140 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| DOEOEBLJ_28 | 38121 | 38639 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
| DOEOEBLJ_11 | 1191425 | 1194022 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DOEOEBLJ_11 | 960555 | 961196 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| DOEOEBLJ_22 | 434015 | 435196 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| DOEOEBLJ_11 | 1242284 | 1244047 | dep/capD | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_389723 |
| DOEOEBLJ_22 | 435679 | 436821 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| DOEOEBLJ_23 | 113115 | 113900 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| DOEOEBLJ_22 | 435211 | 435660 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| DOEOEBLJ_23 | 109279 | 110217 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| DOEOEBLJ_23 | 102125 | 109259 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.22 | 99.97 | NP_391076 |
| DOEOEBLJ_23 | 111893 | 113089 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| DOEOEBLJ_23 | 110245 | 111864 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.07 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DOEOEBLJ_1 | 31800 | 32936 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DOEOEBLJ_3 | 11403 | 10534 | pdxS | 1.14e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DOEOEBLJ_21 | 738028 | 738804 | CodY | 7.65e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DOEOEBLJ_23 | 366263 | 366832 | ClpP | 2.25e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DOEOEBLJ_21 | 275449 | 275841 | spxA1 | 6.02e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DOEOEBLJ_21 | 275449 | 275841 | spx | 3.59e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DOEOEBLJ_2 | 34867 | 34625 | SpoVG | 7.55e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DOEOEBLJ_25 | 145437 | 145634 | CspA | 9.80e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DOEOEBLJ_1 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_2 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_3 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_4 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_5 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_6 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_11 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_21 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_22 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_23 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_24 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_25 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_26 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_27 | 0.0 | 1.0 | 0.0 |
| DOEOEBLJ_28 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DOEOEBLJ_11 | 484819 | 516978 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG0143, VOG4673, VOG4662, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| DOEOEBLJ_11 | 865671 | 876422 | False | Unknown | VOG4548, VOG6332, VOG9957, VOG5532, VOG0052, VOG0026, VOG7462 |
| DOEOEBLJ_11 | 888134 | 914832 | False | Siphoviridae | VOG5129, VOG0713, VOG7884, VOG0052, VOG4581, VOG1886, VOG4556, VOG4573, VOG4553, VOG5601, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4626, VOG4611, VOG4705, VOG0136, VOG4707, VOG8440, VOG0018, VOG6116 |
| DOEOEBLJ_11 | 1004640 | 1016557 | False | Unknown | VOG9712, VOG5042, VOG3389, VOG4910 |
| DOEOEBLJ_21 | 370281 | 397944 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)