Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 2 | 6 | 7.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DBEBKMJH_19 | 27762 | 28808 | clbA | ARO:3002814 | clbA | 92.77 | 99.71 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DBEBKMJH_19 | 27765 | 28808 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.90 | 99.33 | KR610408 |
| DBEBKMJH_21 | 186841 | 188217 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 87.15 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DBEBKMJH_19 | 27765 | 28808 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 92.24 | 99.71 | WP_012116915.1 |
| DBEBKMJH_21 | 186841 | 188211 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.43 | 99.78 | WP_003242953.1 |
| DBEBKMJH_19 | 30559 | 31077 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 82.66 | 100.00 | WP_003242546.1 |
| DBEBKMJH_20 | 5345 | 7942 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DBEBKMJH_11 | 377458 | 378639 | capB | 0.0 | Capsule | Immune modulation | 81.87 | 99.66 | NP_391471 |
| DBEBKMJH_11 | 378654 | 379103 | capC | 3.51e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DBEBKMJH_4 | 27859 | 26723 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| DBEBKMJH_7 | 4404 | 5273 | pdxS | 6.45e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| DBEBKMJH_16 | 400966 | 401742 | CodY | 4.16e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DBEBKMJH_17 | 41683 | 41291 | spxA1 | 4.44e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| DBEBKMJH_19 | 45304 | 45107 | CspA | 3.64e-32 | cold shock protein | 92.42 | 100.00 | CAA62903 |
| DBEBKMJH_15 | 417656 | 417462 | CspD | 1.34e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| DBEBKMJH_15 | 417656 | 417459 | CspB | 2.78e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| DBEBKMJH_19 | 45304 | 45110 | CspR | 1.81e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DBEBKMJH_1 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_2 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_3 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_4 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_5 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_6 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_7 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_8 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_11 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_12 | 1.0 | 0.0 | 1.0 |
| DBEBKMJH_15 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_16 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_17 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_18 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_19 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_20 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_21 | 0.0 | 1.0 | 0.0 |
| DBEBKMJH_22 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DBEBKMJH_11 | 870448 | 880730 | False | Siphoviridae | VOG4551, VOG2786, VOG1563, VOG6546, VOG10937, VOG10564 |
| DBEBKMJH_11 | 892463 | 895077 | False | Unknown | VOG4567, VOG10904 |
| DBEBKMJH_11 | 897318 | 930617 | False | Siphoviridae | VOG7541, VOG0048, VOG0101, VOG2215, VOG0274, VOG1183, VOG0275, VOG5023, VOG3699, VOG2613, VOG0692, VOG4555, VOG3676, VOG5361, VOG2565, VOG11074, VOG5068, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG5221, VOG4824, VOG0602, VOG6116, VOG10051 |
| DBEBKMJH_15 | 34666 | 40185 | False | Siphoviridae | VOG4603, VOG4600, VOG1309, VOG11060, VOG0189 |
| DBEBKMJH_15 | 43984 | 71903 | False | Myoviridae | VOG10057, VOG0796, VOG10227, VOG4773, VOG4688, VOG6480, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG0979, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| DBEBKMJH_16 | 48997 | 77263 | False | Myoviridae | VOG0101, VOG4602, VOG9667, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
| DBEBKMJH_16 | 469004 | 493330 | False | Siphoviridae | VOG0054, VOG8537, VOG10230, VOG4660, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG1637, VOG0275, VOG7930, VOG8610, VOG5008, VOG3390, VOG7718 |
| DBEBKMJH_17 | 302211 | 334132 | False | Siphoviridae | VOG10230, VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0539, VOG0704, VOG4589, VOG0204, VOG3435, VOG4553, VOG4573, VOG4556, VOG4556, VOG4544, VOG0519, VOG0531, VOG7884, VOG0275, VOG0198, VOG6242, VOG6517, VOG6581, VOG0397, VOG8569, VOG4658, VOG5407, VOG1336, VOG6587, VOG6931, VOG0189 |
| DBEBKMJH_18 | 13123 | 27080 | False | Siphoviridae | VOG4602, VOG8483, VOG9667, VOG11075, VOG0650, VOG4552, VOG4600, VOG1309, VOG9093, VOG0189, VOG0044, VOG0536, VOG1336, VOG4658, VOG8569, VOG0397, VOG6581 |
| DBEBKMJH_18 | 29452 | 54776 | False | Siphoviridae | VOG5447, VOG4972, VOG4681, VOG0796, VOG10227, VOG0720, VOG4564, VOG6778, VOG4555, VOG1329, VOG2621, VOG5872, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG4545, VOG4605, VOG4599, VOG3390, VOG5535, VOG0753, VOG0136, VOG4574 |
Phigaro v2.4.0 (Database: Jan. 2024 release)